The purpose of the present study was to investigate the relationship between muscle activation and shooting performance in biathlon at rest and after pysical activity (PA). Seven female age 19 - 24 (21+-1 yrs) regularly competin on natinal/international level participated. The study was made indoor with sharp ammunition and contents of 20 shots at rest an 20 shots at rest and 20 shots after PA (arm-andlegcycle) 4X4 minuters of 85% of HRmax. EMG measured on right, m. Triceps Brachii, m. Deltoideus, m. Flexor Carpi Radialis, m. Extenstor Cari Radialis, left and right m. Bicepts Brachii, left and right m. Gastrocnemiuns, m. Rectus Abdominis. The muscle activation is low for all shooters in all shots at rest and after PA. Left m. Gastrocnemius was more activated in bad shots compared to good at rest (P=0.03). A positive correlation (P<0.05) between good and bad shots shows that muscle activation is not a depending factor in shooting performance. The muscle activation is individual and variations can be explained by altered shooting stance and technique. A stable stance is advantageously in standing shooting. The main results was that if an athlete activate a muscle more or less during bad or good shot is individual.