We propose a topology optimization method for design of transversely isotropic elastic continua subject to high-cycle fatigue. The method is applicable to design of additive manufactured components, where transverse isotropy is often manifested in the form of a lower Young’s modulus but a higher fatigue strength in the build direction. The fatigue constraint is based on a continuous-time model in the form of ordinary differential equations governing the time-evolution of fatigue damage at each point in the design domain. Such evolution occurs when the stress state lies outside a so-called endurance surface that moves in stress space depending on the current stress and a back-stress tensor. Pointwise bounds on the fatigue damage are approximated using a smooth aggregation function, and the fatigue sensitivities are determined by the adjoint method. Several problems where the objective is to minimize mass are solved numerically. The problems involve non-periodic proportional and non-proportional load histories. Two alloy steels, AISI-SAE 4340 and 34CrMo6, are treated and the respective as well as the combined impact of transversely isotropic elastic and fatigue properties on the design are compared.