We investigated the effects of playing surfaces with different impact absorption characteristics on external demand and physiological responses. Fifteen participants completed a soccer match simulation on natural grass, synthetic turf and concrete surfaces. Accelerometry-derived PlayerLoadTM per minute (PL·min−1) and average net force (AvFNet) were used to quantify external demands at the centre of mass (CoM), upper-back, mid-back and hip. Heart rate, oxygen uptake, energy expenditure and RPE quantified physiological responses. The concrete surface exhibited the least impact absorption, with peak decelerations ~3.5x synthetic turf and ~10x natural grass (p < 0.001). Despite this, there was no differences in external demand between surfaces (surface: p ≥ 0.194; η2p≤0.092). Both AvFNet and PL·min−1 (location: p < 0.001; η2p≥0.859) were higher at the hip (613(91)N; 12.5(1.2)arb.u), reduced at the mid-back (521(67)N; 8.8(0.7)arb.u) and upper-back (502(60)N; 8.8(0.7)arb.u) when compared to CoM (576(78)N; 10.7(1.0)arb.u). Although playing surface did not influence the external demands, heart rate or oxygen uptake (p > 0.05), energy expenditure was highest on natural grass compared to synthetic turf (P = 0.034) and RPE was highest on synthetic turf compared to concrete (p = 0.026). Different playing surfaces can alter physiological responses to soccer-specific activity even when the external demands are similar.