Purpose: To compare the frequency and severity of self-perceived negative symptoms associated with the menstrual cycle or hormonal-contraceptive (HC) cycle between (1) HC users and non–HC users and (2) different competition levels. An additional aim was to describe the rate and type of HC use and prevalence of potential menstrual-cycle disturbances in female team handball players. Methods: A total of 136 team handball players, competing at different levels (international, 48; national, 52; local/regional, 36), completed a cross-sectional survey regarding self-reported negative cycle-related symptom frequency and severity, perceived influence on training/competition performance, menstrual function, and current and/or past HC use. Results: Although most players regularly experienced negative cycle-related symptoms, non–HC users reported more frequent and severe cramps (P = .001 to .026), as well as a higher occurrence of fatigue and skin problems (P = .019 to .045), compared with HC users. Negative symptoms such as fatigue were more common and severe for local/regional-level players compared with players at higher (international) competition levels (P = .006–.046). Half (47%) of the players currently used HC, primarily long-acting reversible contraceptives (52%), as well as the combined oral (33%) or progestin-only pill (14%). Notably, only 2% of players altered their training in response to negative cycle-related symptoms, despite 41% perceiving that these symptoms interfered with training quality and athletic performance. Conclusions: Negative cycle-related symptoms are common among team handball players, regardless of HC use, and are perceived by many players to adversely influence their athletic performance.