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  • 1. Berglund, P.
    et al.
    Hedenström, Erik
    Mid Sweden University, Faculty of Science, Technology and Media, Department of Natural Sciences.
    Hult, K.
    Controlling lipase enantioselectivity for organic synthesis: Meeting abstract2000In: ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2000, Vol. 219, p. U219-Conference paper (Refereed)
  • 2.
    Essén, Sofia
    Mid Sweden University, Faculty of Science, Technology and Media, Department of Natural Sciences.
    Hydroxamate Siderophores and Low Molecular Mass Organic Acids in Terrestrial and Aquatic Environments Determined by Liquid Chromatography Mass Spectrometry2007Doctoral thesis, comprehensive summary (Other academic)
    Abstract [en]

    Siderophores are low molecular mass organic compounds with high iron(III) stability constants. They are produced by many microorganisms, often in combination with low molecular mass organic acids (LMMOAs). Both siderophores and LMMOAs accelerate mineral weathering, thereby enhancing mineral nutrient availability. Siderophore concentrations in natural environments, however, are largely unknown. In the present work, analytical methods based on liquid chromatography (LC) electrospray ionization mass spectrometry (ESI‐MS) were developed and used for concentration determination of selected hydroxamate siderophores and LMMOAs. The use of capillary LC with on‐line pre‐concentration via column switching enabled siderophore quantification with detection limits of 0.1 ‐ 5 nM. Through solid phase extraction even lower detection limits were achieved. In Swedish podzol soil solutions concentrations up to 12 nM ferricrocin and 2 nM ferrichrome were found in the upper soil layes. Additionally, in axenic pine (Pinus sylvestris) seedling cultures, the ectomycorrhizal fungus Hebeloma crustuliniforme was shown to produce ferricrocin as the main siderophore. Moreover, ferricrocin was found in enriched Baltic Sea cyanobacterial populations at concentrations up to 1 nM, while concentrations up to 0.05 nM ferrioxamine B were determined in Baltic Sea surface waters during cyanobacterial blooms. Siderophores have strong affinities for some actinides and may affect radionuclide mobilization in deep subsurface nuclear wase repositories. Two subsurface bacterial strains were shown to produce siderophores under aerobic conditions, i.e. Pseudomonas stutzeri produced mainly ferrioxamine E and Pseudomonas fluorescens produced one pyoverdin siderophore. Siderophores produced by aerobically grown P. stutzeri were not observed either in anaerobic cultures or in deep subsurface water samples.

  • 3.
    Essén, Sofia
    et al.
    Mid Sweden University, Faculty of Science, Technology and Media, Department of Natural Sciences, Engineering and Mathematics.
    Bylund, Dan
    Mid Sweden University, Faculty of Science, Technology and Media, Department of Natural Sciences, Engineering and Mathematics.
    Walve, J
    Ingri, J
    Lundström, Ulla S
    Mid Sweden University, Faculty of Science, Technology and Media, Department of Natural Sciences, Engineering and Mathematics.
    Quantification of hydroxamate siderophores in the Baltic Sea and cyanobacterial populations by capillary LC-ESI-MS/MSManuscript (Other (popular science, discussion, etc.))
  • 4.
    Fredriksson, Mattias
    et al.
    Mid Sweden University, Faculty of Science, Technology and Media, Department of Natural Sciences, Engineering and Mathematics.
    Petersson, Patrik
    Axelsson, Bengt-Olof
    Bylund, Dan
    Mid Sweden University, Faculty of Science, Technology and Media, Department of Natural Sciences, Engineering and Mathematics.
    An automatic peak finding method for LC-MS data using Gaussian second derivative filtering2009In: Journal of Separation Science, ISSN 1615-9306, E-ISSN 1615-9314, Vol. 32, no 22, p. 3906-3918Article in journal (Refereed)
    Abstract [en]

    A highly automated procedure for localising and characterising peaks in the chromatographic time domain of LC-MS data has been developed. The work was initiated by an identified need to facilitate the detection and tracking of chromatographic peaks during method development for the analysis of impurities in pharmaceutical products. The algorithm is mainly based on a digital filter for which the settings are automatically adapted to the data set under study. The procedure was evaluated for synthetic data sets with various S/N levels, peak widths and baseline properties. It was found that even for the worst case tested with S/N=10 and a high variability in the baseline, 94% of the simulated analytical peaks could be detected without producing any false-positive identifications. Furthermore, the number of correctly estimated peak heights and peak widths falling within a 10% error of the true values were 94 and 91%, respectively. For experimental data sets, peak height, and width estimations were more difficult, but the processed reconstructions showed an excellent agreement with the analytical signals of the raw data, and also a clearly improved visualisation in total ion- and base-peak chromatograms.

  • 5.
    Fäldt, J.
    et al.
    Royal Institute of Technology, Department of Chemistry, Organic Chemistry, Group of Ecological Chemistry, SE-100 44 Stockholm.
    Sjödin, Kristina
    Mid Sweden University, Faculty of Science, Technology and Media, Department of Natural Sciences.
    Persson, M.
    Royal Institute of Technology, Department of Chemistry, Organic Chemistry, Group of Ecological Chemistry, SE-100 44 Stockholm.
    Valterová, I.
    Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Flemingovo nám. 2, 166 10 Prague 6, Czech Republic.
    Borg-Karlson, Anna-Karin
    Royal Institute of Technology, Department of Chemistry, Organic Chemistry, Group of Ecological Chemistry, SE-100 44 Stockholm.
    Correlations between selected monoterpene hydrocarbons in the xylem of six Pinus (pinaceae) species2001In: Chemoecology, ISSN 0937-7409, E-ISSN 1423-0445, Vol. 11, no 2, p. 97-106Article in journal (Refereed)
    Abstract [en]

    The compositions of 23 monoterpene hydrocarbons of six pine species (Pinus sylvestris, P. yunnanensis ll,ris, P. armandii, P. tropicalis, P. cubensis and P. caribaca) were compared, using multivariate data analysis. Four of the six species were clearly different from the other species in a Principal Components Analysis (PCA) model, based on the relative amounts (selective normalization) of the monoterpenes. The correlation coefficients between constituents were determined separately for each species and the strongest correlations were found between (+)-alpha -pinene and (+)-camphene and between the corresponding (-)-enantiomers, in all species. This pattern, i.e. a good correlation in all species, was neither shown by the correlation of the structurally more similar (+)-alpha/beta -pinenes, nor by the (-)-alpha/beta -pinenes or within the enantiomeric pairs of alpha -pinene and beta -pinene. For these pairs of monoterpenes, good correlations were found in some species. None of the species showed good correlations in all the investigated monoterpene pairs presented here. Correlations between monoterpenes in insect-attacked trees (P. cubensis and P. caribaea, attacked by Dioryctria horneana, and P. yunnanensis, attacked by Tomicus piniperda) were also determined. The results are discussed from chemotaxonomic and biosynthetic points of view.

  • 6.
    Holmström, Sara J M
    et al.
    Mid Sweden University, Faculty of Science, Technology and Media, Department of Natural Sciences, Engineering and Mathematics.
    Rosling, A
    Finlay, R D
    van Hees, P A W
    Lundström, Ulla
    Mid Sweden University, Faculty of Science, Technology and Media, Department of Natural Sciences, Engineering and Mathematics.
    Contribution of ectomycorrhizal fungi to biogeochemical processes during iron and calcium limitation: Meeting abstract2009In: GEOCHIMICA ET COSMOCHIMICA ACTA, Pergamon Press, 2009, p. A546-A546Conference paper (Refereed)
  • 7. Nieberding, Caroline. M.
    et al.
    de Vos, Helena
    Schneider, Maria V.
    Lassance, Jean-Marc
    Estramil, Natalia
    Andersson, Jimmy
    Mid Sweden University, Faculty of Science, Technology and Media, Department of Natural Sciences, Engineering and Mathematics.
    Bång, Joakim
    Mid Sweden University, Faculty of Science, Technology and Media, Department of Natural Sciences, Engineering and Mathematics.
    Hedenström, Erik
    Mid Sweden University, Faculty of Science, Technology and Media, Department of Natural Sciences, Engineering and Mathematics.
    Löfstedt, Christer
    Brakefield, Paul M.
    The Male Sex pheromone of the Butterfly Bicyclus anynana: Towards an Evolutionary Analysis2008In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 3, no 7, p. e2751-Article in journal (Refereed)
    Abstract [en]

    Background: Female sex pheromones attracting mating partners over long distances are a major determinant of reproductive isolation and speciation in Lepidoptera. Males can also produce sex pheromones but their study, particularly in butterflies, has received little attention. A detailed comparison of sex pheromones in male butterflies with those of female moths would reveal patterns of conservation versus novelty in the associated behaviours, biosynthetic pathways, compounds, scent-releasing structures and receiving systems. Here we assess whether the African butterfly Bicyclus anynana, for which genetic, genomic, phylogenetic, ecological and ethological tools are available, represents a relevant model to contribute to such comparative studies.

    Methodology/Principal Findings: Using a multidisciplinary approach, we determined the chemical composition of the male sex pheromone (MSP) in the African butterfly B. anynana, and demonstrated its behavioural activity. First, we identified three compounds forming the presumptive MSP, namely (Z)-9-tetradecenol (Z9-14:OH), hexadecanal (16:Ald) and 6,10,14-trimethylpentadecan-2-ol (6,10,14-trime-15-2-ol), and produced by the male secondary sexual structures, the androconia. Second, we described the male courtship sequence and found that males with artificially reduced amounts of MSP have a reduced mating success in semi-field conditions. Finally, we could restore the mating success of these males by perfuming them with the synthetic MSP.

    Conclusions/Significance: This study provides one of the first integrative analyses of a MSP in butterflies. The toolkit it has developed will enable the investigation of the type of information about male quality that is conveyed by the MSP in intraspecific communication. Interestingly, the chemical structure of B. anynana MSP is similar to some sex pheromones of female moths making a direct comparison of pheromone biosynthesis between male butterflies and female moths relevant to future research. Such a comparison will in turn contribute to understanding the evolution of sex pheromone production and reception in butterflies.

  • 8.
    Norström, Sara H
    Mid Sweden University, Faculty of Science, Technology and Media, Department of Natural Sciences, Engineering and Mathematics.
    Soil and stream water chemistry in a boreal catchment - interactions, influences of dissolved organic matter and effects of wood ash application2010Doctoral thesis, comprehensive summary (Other academic)
    Abstract [en]

    Two small bordering catchments in Bispgården, Central Sweden, wereinvestigated in regard to soil solution and stream water chemistry during the frostfree seasons of 2003-2007. Both catchments were drained by first order streams,Fanbergsbäcken and Gråbergsbäcken, and in Fanbergsbäckens catchment anextensive investigation of the soil and soil solution chemistry was conducted bylysimeter and centrifugation sampling. The area of intensive soil solutioninvestigation was situated in a slope towards a stream incorporating a rechargearea, with podzolic soil, and a discharge area close to the stream with an arenosolsoil. Samples were continuously taken in both the recharge- and the discharge areaof the slope, and stream water was sampled in the streams of both catchments. Themain variables of interest of the study were the interactions, the influence ofdissolved organic carbon and the effects of wood ash application to soil solutionand stream water.The natural variations and the interactions between soil solution and streamwater were monitored during 2003-2004. In soil solution, most of the investigatedsubstances tended to increase during the growing season, due to weathering andmicrobial degradation of biota. Ca, Mg, Al and Fe were highly associated todissolved organic carbon (DOC) throughout the catchment. The low molecularfraction of DOC seemed to have a higher impact on the soil processes in therecharge area, while high molecular DOC was more important for transport ofcations in the discharge area and the stream water.The concentration of different substances in the two streams differedsignificantly, even though the catchments were similar in size, shape andforestation. The seasonal patterns of most of the substances measured weresignificantly correlated between the streams, however. Cations and pH correlatedwell with DOC and flow. The flow pattern driven by precipitation seems to be thedriver of the stream water chemistry.Wood ash was applied at a dosage of 3 ton/ha to one of the catchments in theautumn of 2004, to investigate the initial effects on the soil solution- and streamwater chemistry. WAA is recommended by the Swedish Forest Agency tocounteract acidification in soil and runoff that may be caused by an intensivebiomass harvesting. The impact of the WAA was studied during 2005-2006.Compared to the control temporarily higher concentrations of K, Ca and SO4 wereobserved in the soil solution of the ashed area. In the stream water the effects of theWAA were easier to distinguish due to higher sampling frequency. The strongesteffect was seen for K, but increases in the stream water were also noted for DOC,Ca, Mg, Si, Cl and malonate. No increase in pH could be statistically verifiedhowever, and overall the initial effects of the WAA seem mild.

  • 9.
    Norström, Sara H
    et al.
    Mid Sweden University, Faculty of Science, Technology and Media, Department of Natural Sciences, Engineering and Mathematics.
    Vestin, Jenny LK
    Swedish geotechnical institute.
    Bylund, Dan
    Mid Sweden University, Faculty of Science, Technology and Media, Department of Natural Sciences, Engineering and Mathematics.
    Lundström, Ulla S
    Mid Sweden University, Faculty of Science, Technology and Media, Department of Natural Sciences, Engineering and Mathematics.
    Influences of dissolved organic carbon on stream water chemistry in two forested catchments in central Sweden2010In: Biogeochemistry, ISSN 0168-2563, E-ISSN 1573-515X, Vol. 101, no 1-3, p. 229-241Article in journal (Refereed)
    Abstract [en]

    Stream water chemistry in two headwater streams draining two small, adjacent catchments in Bispgården, central Sweden was studied during the 2003 and 2004 growing seasons. The two catchments closely resemble each other in regard to size, shape and drainage density, with the major difference found in the area of wetland lining the streambeds. The emphasis of the study was to investigate the stream water chemistry of these closely resembling catchments, regarding the quality and quantity of dissolved organic carbon (DOC) and its influence on the concentrations of di- and trivalent cations. The streams showed significant differences in the content and size distribution of DOC and in the distribution of cations between the different size fractions. For both streams the high flow events induced by precipitation influenced the chemistry of the streams through increase of organic matter and its associated cations. Fanbergsbäcken, with relatively low pH and high DOC concentration, had a greater amount of high molecular mass (HMM) DOC to which approximately 75% of Al and Fe and about 50% of Ca and Mg were associated. Gråbergsbäcken, with a higher pH and lower DOC level, had approximately 65% of Al, 40% of Fe and 30% of Ca and Mg associated to its HMM DOC fraction. Sixteen different low molecular mass organic acids were found in the stream water, of which oxalic and lactic acid were present in the highest concentrations.

  • 10. Persson, Per Valdemar
    et al.
    Schröder, Jessica
    Mid Sweden University, Faculty of Science, Technology and Media, Department of Natural Sciences.
    Wickholm, Kristina
    Hedenström, Erik
    Mid Sweden University, Faculty of Science, Technology and Media, Department of Natural Sciences.
    Iversen, Tommy
    Selective organocatalytic ring-opening polymerization: A versatile route to carbohydrate-functionalized poly(e-caprolactenes)2004In: Macromolecules, ISSN 0024-9297, E-ISSN 1520-5835, Vol. 37, no 16, p. 5889-5893Article in journal (Refereed)
    Abstract [en]

    Cationic catalysis using simple carboxylic acids to combine the ring-opening polymerization of c-caprolactone and the regioselective acylation of carbohydrates has been investigated. L-Lactic acid catalyzed the acylation of methyl beta-D-glucopyranoside and sucrose with epsilon-caprolactone in high yield by bulk polymerization at 120 degreesC. The main products were regioselectively acylated on the primary hydroxyl groups of the carbohydrate end groups. The overall conversion to methyl beta-D-glucopyranoside-functionalized poly(epsilon-caprolactone) was more than 90%, M-w 2000 and polydispersity index 1.5, with one major product methyl 6-O-poly(epsilon-caprolactone)-beta-D-glucopyranoside, in agreement with the corresponding Candida antarctica lipase B-catalyzed acylation

  • 11.
    Schröder, Jessica
    Mid Sweden University, Faculty of Science, Technology and Media, Department of Natural Sciences.
    Synthesis of End-functionalized Polyesters byRing-Opening Polymerization of ε-Caprolactone2004Licentiate thesis, comprehensive summary (Other academic)
    Abstract [en]

    The main objective of this work was to investigate the ring-opening polymerizations

    of cyclic esters by enzyme catalysis and cationic activated monomer polymerization

    in the search for new polymer materials and fiber-based composites. Candida

    antarcica lipase B was used for end-functionalization of poly(ε-caprolactone) and in

    the copolymerization of D,L-lactide and ε-caprolactone. The reactions were

    characterized with regard to polymer structure and molecular weight distributions by

    NMR, MALDI-TOF MS and GPC.

    The copolymerization of D,L-lactide and ε-caprolactone initially leads to a

    nonrandom incorporation of lactide. D,L-Lactide is incorporated as the dimer in the

    initial stage, when ε-caprolactone is practically excluded from the reaction. During

    this stage, the polymerization is slowed by the presence of D,L-lactide as compared to

    a neat ε-caprolactone polymerization. After the initial stage, ε-caprolactone and

    lactide are polymerized at approximately equal rates.

    In cationic activated monomer polymerizations of ε-caprolactone, lactic acid can act

    as initiator, catalyst and terminator. Molecular weights distributions and reaction

    times are comparable with the corresponding lipase-catalyzed reactions. If more

    nucleophilic initiators, e.g. methyl β-D-glucopyranoside, sucrose or raffinose, are

    present in the reaction mixture, lactic acid catalyzes the reaction without participating

    in the initiation. The regioselectivity of the acylation is well agreement with the

    corresponding lipase catalyzed reaction.

    Both methods have several advantages: the catalysts can be handled without need

    for extraordinary precautions with regard to atmosphere, humidity or equipment. The

    polymerizations are performed under mild reaction conditions, with non-toxic

    catalysts that can be recycled, and show the possibility of controlling end-group

    functionalization with high precision.

  • 12.
    Sukhovey, Yurij G.
    et al.
    Institute of Clinical Immunology, Siberian Division of Russian Academy of Sciences, Tyumen, Russia.
    Koptyug, Andrey
    Mid Sweden University, Faculty of Science, Technology and Media, Department of Quality Technology and Management, Mechanical Engineering and Mathematics. SportsTech Research Centre, Mid Sweden University.
    Fisher, Tatjana A.
    Tyumen Research Centre, Siberian Division of Russian Academy of Sciences, Tyumen, Russia.
    Petrov, Sergey A.
    Tyumen State Oil and Gas Institute, Volodarskogo st. 38, , 625000, Tyumen, .
    Dotsenko, Evgenij L.
    Tyumen State University, Semakova st. 10, 625003, Tyumen, Russia.
    Functional Conjugation of the Different Regulatory Responses to the Stress Stimuli in Healthy Human Subjects2016In: Open Journal of Applied Sciences, ISSN 2165-3917, Vol. 6, p. 489-500Article in journal (Refereed)
    Abstract [en]

    Present article discusses the physiological mechanisms of the state employees adaptation duringactive training in temporary groups. It is suggested that adaptive mechanisms to adverse effectsmay be studied basing on the concept of functional isomorphism of the psychic and immune systems.Adaptive mechanisms were studied through the monitoring of the stress factors’ impact upon thelaw enforcement officers when training outside the places of permanent deployment. The specificpurpose of present study was to evaluate the physiological indicators of the psychic, immune andendocrine systems dynamics at different stages of adaptation of the live organism to a stressfulsituation, hoping to get better insight into possible relations between psychic and immune domains.Through monitoring of the dynamics of the endocrine and immune responses to the psychic stimuli,it was possible to correlate the stages of the stress onset to the phases of specific immune reactions.Strong correlations between the parameters characterizing activation of the psychic and immuneresponses support the hypothesis of the presence of “strong cooperation” between psychic andimmune domains. It supports earlier hypothesis that we are monitoring

  • 13.
    Sunnerheim, Kerstin
    et al.
    Mid Sweden University, Faculty of Science, Technology and Media, Department of Natural Sciences.
    Bratt, Katharina
    Identification of centrolobol as the platyphylloside metabolite responsible for the observed effect on in vitro digestibility of hay.2004In: Journal of Agricultural and Food Chemistry, ISSN 0021-8561, E-ISSN 1520-5118, Vol. 52, no 19, p. 5869-5872Article in journal (Refereed)
    Abstract [en]

    Syntheses of the metabolites from platyphylloside, a phenol causing digestibility inhibition in rumen fluid, have been performed to identify the active metabolite. 1,7-Bis(4‘-hydroxyphenyl)-3-heptanone (3-platyphyllone), racemic, and the two enantiomers of 1,7-bis(4‘-hydroxyphenyl)-3-heptanol (centrolobol) and 1,7-bis(4-hydroxyphenyl)heptane (platyphyllane) were synthesized and tested regarding digestibility inhibition in vitro in cow rumen fluid. All compounds tested induced a decreased digestion. Centrolobol was found to be the metabolite causing the observed effect, and (R)-centrolobol was found to be the enantiomer formed in the rumen liquor in vitro.

  • 14.
    Vestin, Jenny
    Mid Sweden University, Faculty of Science, Technology and Media, Department of Natural Sciences.
    Biogeochemical Interactions between Soil, Soil Solution and Stream Water2007Doctoral thesis, comprehensive summary (Other academic)
  • 15.
    Östberg, Tomas L
    et al.
    Mid Sweden University, Faculty of Science, Technology and Media, Department of Natural Sciences.
    Jonsson, A P
    Lundström, Ulla
    Mid Sweden University, Faculty of Science, Technology and Media, Department of Natural Sciences.
    Bylund, Dan
    Mid Sweden University, Faculty of Science, Technology and Media, Department of Natural Sciences.
    Effects of whey addition on the biodegradation of n-hexadecane, n-octadecane and phenanthrene in diesel fuel contaminated soilManuscript (preprint) (Other academic)
1 - 15 of 15
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