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Repeated high-intensity exercise modulates Ca2+ sensitivity of human skeletal muscle fibers
Univ Southern Denmark, SDU Muscle Res Cluster, Dept Sports Sci & Clin Biomech, Campusvej 55, DK-5230 Odense M, Denmark.
Univ Southern Denmark, SDU Muscle Res Cluster, Dept Sports Sci & Clin Biomech, Campusvej 55, DK-5230 Odense M, Denmark.
Mid Sweden University, Faculty of Human Sciences, Department of Health Sciences. (NVC)
Mid Sweden University, Faculty of Human Sciences, Department of Health Sciences. (NVC)
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2016 (English)In: Scandinavian Journal of Medicine and Science in Sports, ISSN 0905-7188, E-ISSN 1600-0838, Vol. 26, no 5, 488-497 p.Article in journal (Refereed) Published
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Abstract [en]

The effects of short-term high-intensity exercise on single fiber contractile function in humans are unknown. Therefore, the purposes of this study were: (a) to access the acute effects of repeated high-intensity exercise on human single muscle fiber contractile function; and (b) to examine whether contractile function was affected by alterations in the redox balance. Eleven elite cross-country skiers performed four maximal bouts of 1300m treadmill skiing with 45min recovery. Contractile function of chemically skinned single fibers from triceps brachii was examined before the first and following the fourth sprint with respect to Ca2+ sensitivity and maximal Ca2+-activated force. To investigate the oxidative effects of exercise on single fiber contractile function, a subset of fibers was incubated with dithiothreitol (DTT) before analysis. Ca2+ sensitivity was enhanced by exercise in both MHC I (17%, P<0.05) and MHC II (15%, P<0.05) fibers. This potentiation was not present after incubation of fibers with DTT. Specific force of both MHC I and MHC II fibers was unaffected by exercise. In conclusion, repeated high-intensity exercise increased Ca2+ sensitivity in both MHC I and MHC II fibers. This effect was not observed in a reducing environment indicative of an exercise-induced oxidation of the human contractile apparatus.

Place, publisher, year, edition, pages
2016. Vol. 26, no 5, 488-497 p.
Keyword [en]
Sprint skiing, muscle fiber, specific force, fatigue
National Category
Sport and Fitness Sciences
Identifiers
URN: urn:nbn:se:miun:diva-27809DOI: 10.1111/sms.12483ISI: 000375077800002PubMedID: 25944268Scopus ID: 2-s2.0-84946189228OAI: oai:DiVA.org:miun-27809DiVA: diva2:934320
Available from: 2016-06-08 Created: 2016-06-07 Last updated: 2016-09-22Bibliographically approved

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Willis, Sarah J.Andersson, ErikHolmberg, Hans-Christer
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