miun.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Smoking Induces Overexpression of Immediate Early Genes in Active Graves' Ophthalmopathy
Department of Clinical Sciences, Diabetes and Endocrinology, CRC, Skane University Hospital, Jan Waldenströms gata 35, Malmö, Sweden.
Department of Clinical Sciences Malmö, Lunds Universitet, Lund, Sweden.
YouGenomics LLC, Germantown, MD, United States .
Mid Sweden University, Faculty of Human Sciences, Department of Health Sciences. YouGenomics LLC, Germantown, MD, United States . (NVC)
Show others and affiliations
2014 (English)In: Thyroid, ISSN 1050-7256, E-ISSN 1557-9077, Vol. 24, no 10, p. 1524-1532Article in journal (Refereed) Published
Abstract [en]

Background: Cigarette smoking is a risk factor for the development of Graves' ophthalmopathy (GO). In a previous study of gene expression in intraorbital fat, adipocyte-related immediate early genes (IEGs) were overexpressed in patients with GO compared to controls. We investigated whether IEGs are upregulated by smoking, and examined other pathways that may be affected by smoking. Methods: Gene expression in intraorbital fat was studied in smokers (n=8) and nonsmokers (n=8) with severe active GO, as well as in subcutaneous fat in thyroid-healthy smokers (n=5) and nonsmokers (n=5) using microarray and real-time polymerase chain reaction (PCR). Results: With microarray, eight IEGs were upregulated more than 1.5-fold in smokers compared to nonsmokers with GO. Five were chosen for confirmation and were also overexpressed with real-time PCR. Interleukin-1 beta/IL-1B/(2.3-fold) and interleukin-6/IL-6/(2.4-fold) were upregulated both with microarray and with real-time PCR in smokers with GO compared to nonsmokers. Major histocompatibility complex, class II, DR beta 1/HLA-DRB1/was upregulated with microarray (2.1-fold) and with borderline significance with real-time PCR. None of these genes were upregulated in smokers compared to nonsmokers in subcutaneous fat. Conclusions: IEGs, IL-1B, and IL-6 were overexpressed in smokers with severe active GO compared to nonsmokers, suggesting that smoking activates pathways associated with adipogenesis and inflammation. This study underlines the importance of IEGs in the pathogenesis of GO, and provides evidence for possible novel therapeutic interventions in GO. The mechanisms activated by smoking may be shared with other conditions such as rheumatoid arthritis.

Place, publisher, year, edition, pages
2014. Vol. 24, no 10, p. 1524-1532
National Category
Endocrinology and Diabetes
Identifiers
URN: urn:nbn:se:miun:diva-23439DOI: 10.1089/thy.2014.0153ISI: 000342742800012Scopus ID: 2-s2.0-84907438895OAI: oai:DiVA.org:miun-23439DiVA, id: diva2:778273
Available from: 2015-01-09 Created: 2014-11-17 Last updated: 2017-12-05Bibliographically approved

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full textScopus

Authority records BETA

Ström, Kristoffer

Search in DiVA

By author/editor
Ström, Kristoffer
By organisation
Department of Health Sciences
In the same journal
Thyroid
Endocrinology and Diabetes

Search outside of DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 471 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf