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Strong clustering in a SNP study of Phlebiopsis gigantea in sweden
Mid Sweden University, Faculty of Science, Technology and Media, Department of Natural Sciences. (Biologi)
Mid Sweden University, Faculty of Science, Technology and Media, Department of Natural Sciences.
(English)Manuscript (preprint) (Other academic)
Abstract [en]

The population structures of wood decaying basidomycetes depend on several factors; one is dispersal pattern of spores, another is age structure including lifespan and also environmental impacts like forest fires. Phlebiopsis gigantea has been shown to be in the group of basidomycetes that have a well developed tolerance to heat, is long-distance wind dispersed and whose fruit body show up early in succession on fallen logs. In a study of 132 individuals from 3 pairs of locations, 350 km apart, in middle to northern Sweden we used 26 SNP-markers in 6 loci to make a genetic clustering study using STRUCTURE (v. 2.3.4.). The hypothesis; first, clustering should follow the geographic sampling locations with more gene flow between geographically close locations, second; that genetic distance between different clusters should be low due to the long distance dispersal of spores, third; as markers are random we don’t expect to find a correlation between locations affected by forest fires and locations not affected by forest fires. In the study we found 5 clusters (Pr[K= 1]) with moderate to high Fst values (0,0697-03939). Clusters had a poor geographical correlation to sampled populations indicating a complicated population structure. Out of 132 individuals 119 had a private genotype showing a large genetic variation over the total area and a low level of clones in the field.         

National Category
Genetics
Identifiers
URN: urn:nbn:se:miun:diva-23060OAI: oai:DiVA.org:miun-23060DiVA: diva2:749428
Available from: 2014-09-24 Created: 2014-09-24 Last updated: 2014-09-25Bibliographically approved
In thesis
1. Wood Fungi and Forest Fire
Open this publication in new window or tab >>Wood Fungi and Forest Fire
2014 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [sv]

Forest fires have been the major stand-replacing/modifying disturbance in boreal forests. To adapt to fire disturbance, different strategies have evolved. This thesis focuses on wood fungi, and the effect of forest fire on this organism group. In many ways it is a study on adaptation to forest fire, in concurrence with adaptation to dry open habitats. In Paper I we study increased heat resistance in  mycelia from species prevalent in fire prone environments. Fungi were cultivated on fresh wood and exposed to different temperatures. Species prevalent in fire affected habitats had a much higher survival rate over all combinations of time and temperature compared to species associated with other environments. Based on this results the competitiveness was tested after temperature stress (paper II), three fire associated species, were tested against three non fire associated species. All fire associated species had a clear advantage after heat treatment, conquering a larger volume of wood than its competitor. In paper III we studied the effect of heat shock on decomposition rate, 18 species was tested. Species were cultivated and monitored for CO2 accumulation for 8 weeks and then heat shocked. All species including non fire associated species seemed to up-regulate decomposition after heat shock, this response was more pronounced in fire associated species. To look at the possible effect of forest fire on population structure (Paper IV), we developed 29 SNP/INDELs for Phlebiopsis. gigantea. We amplified the marker containing fragments in 132 individuals of P. gigantea in 6 populations, 3 which were found in areas affected by forest fire and 3 in unaffected areas. We found no genetic structure in accordance to forest fire. However we detected geographic structure, which stands in contrast to earlier studies. This might be due to the method, using SNP´s and number of individuals in the study. Finally we collected cross-sections of decayed logs to evaluate the number of fungal species domains that are likely to be hit when drilling a saw-dust sample in a log. We used these estimates to simulate how many species that will be found by a certain number of samples. We found that in 99% of the

Place, publisher, year, edition, pages
Sundsvall: Mid Sweden University, 2014. 178 p.
Series
Mid Sweden University doctoral thesis, ISSN 1652-893X ; 204
National Category
Biological Sciences
Identifiers
urn:nbn:se:miun:diva-23062 (URN)978-91-87557-88-0 (ISBN)
Public defence
2014-10-03, M111, Holmgatan 10, Sundsvall, 11:22 (English)
Opponent
Supervisors
Available from: 2014-09-25 Created: 2014-09-24 Last updated: 2015-03-13Bibliographically approved

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