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Extraction of Antioxidants from Spruce (Picea abies) Bark using Eco-friendly Solvents
Dept Phys & Analyt Chem, Uppsala Univ, POB 599, SE-75124 Uppsala.
Mid Sweden University, Faculty of Science, Technology and Media, Department of applied science and design.
Dept Biochem & Organ Chem, Uppsala Univ, SE-75123 Uppsala.
Mid Sweden University, Faculty of Science, Technology and Media, Department of applied science and design.
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2012 (English)In: Phytochemical Analysis, ISSN 0958-0344, E-ISSN 1099-1565, Vol. 23, no 1, 1-11 p.Article in journal (Refereed) Published
Abstract [en]

Introduction - Antioxidants are known to avert oxidation processes and they are found in trees and other plant materials. Tree bark is a major waste product from paper pulp industries; hence it is worthwhile to develop an extraction technique to extract the antioxidants. Objective - To develop a fast and environmentally sustainable extraction technique for the extraction of antioxidants from bark of spruce (Picea abies) and also to identify the extracted antioxidants that are abundant in spruce bark. Methodology - A screening experiment that involved three different techniques was conducted to determine the best technique to extract antioxidants. The antioxidant capacity of the extracts was determined with DPPH (2,2-diphenyl-1-picrylhydrazyl) assay. Pressurised fluid extraction (PFE) turned out to be the best technique and a response surface design was therefore utilised to optimise PFE. Furthermore, NMR and HPLC-DAD-MS/MS were applied to identify the extracted antioxidants. Results - PFE using water and ethanol as solvent at 160 and 180°C, respectively, gave extracts of the highest antioxidant capacity. Stilbene glucosides such as isorhapontin, piceid and astringin were identified in the extracts. Conclusion - The study has shown that PFE is a fast and environmentally sustainable technique, using water and ethanol as solvent for the extraction of antioxidants from spruce bark. Copyright © 2011 John Wiley and Sons, Ltd. This paper includes four tables in which the extracted solid yield and the antioxidant capacity of spruce bark extracts were presented for the different extraction techniques, as well as the response surface coefficients for the optimised PFE were presented. © 2011 John Wiley and Sons, Ltd..

Place, publisher, year, edition, pages
2012. Vol. 23, no 1, 1-11 p.
Keyword [en]
Accelerated solvent extraction, Antioxidant, DPPH, Ethanol, Picea abies, Pressurised fluid extraction, Stilbene glucosides, Supercritical fluid extraction, Water
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:miun:diva-14214DOI: 10.1002/pca.1316ISI: 000298260100001Scopus ID: 2-s2.0-82955198459OAI: oai:DiVA.org:miun-14214DiVA: diva2:431445
Available from: 2011-07-19 Created: 2011-07-19 Last updated: 2015-01-02Bibliographically approved
In thesis
1. Naturally occurring phenols with antioxidant, antifungal and anti-browsing activity
Open this publication in new window or tab >>Naturally occurring phenols with antioxidant, antifungal and anti-browsing activity
2014 (English)Doctoral thesis, comprehensive summary (Other academic)
Place, publisher, year, edition, pages
Sundsvall: Mid Sweden University, 2014. 68 p.
Series
Mid Sweden University doctoral thesis, ISSN 1652-893X ; 198
National Category
Chemical Sciences
Identifiers
urn:nbn:se:miun:diva-24028 (URN)978-91-87557-77-4 (ISBN)
Public defence
2014-09-26, 11:00
Supervisors
Available from: 2015-01-02 Created: 2015-01-02 Last updated: 2015-03-13Bibliographically approved

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