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Phase behaviour of the 1-monooleoyl-rac-glycerol/n-octyl-beta-D-glucoside/water system
2004 (English)In: TRENDS IN COLLOID AND INTERFACE SCIENCE XVI / [ed] Miguel, MG; Burrows, HD, Springer, 2004, Vol. 123, 36-39 p.Conference paper, Published paper (Refereed)
Abstract [en]

Obtaining high-quality crystals for X-ray diffraction from membrane proteins has proven to be a difficult task. One recently presented method utilises the cubic phases formed by 1-monooleoyl-rac-glycerol (MO). Removing the proteins from their native environment requires the use of surfactants. One commonly used surfactant is n-octyl-beta-D-glucopyranoside (OG). Using NMR techniques and visual observations, the ternary phase diagram of MO/OG/(H2O)-H-2 was outlined at 25 degreesC. The preliminary data show that all phases present in the binary systems at this temperature are also found in the ternary. Further, at the OG-rich side, an additional phase that appears to be hexagonal occurs. Addition of minor amounts (approximate to1.5 wt/wt %) of OG converts the cubic phases of MO to a lamellar structure, while the OG-rich cubic phase is able to dissolve about 15 wt/wt % MO. OG in water forms a large micellar solution phase;. Increasing the MO concentration at constant water content leads to a series of two- and three-phase areas in which one or two phases are in equilibrium with almost pure water.

Place, publisher, year, edition, pages
Springer, 2004. Vol. 123, 36-39 p.
Series
PROGRESS IN COLLOID AND POLYMER SCIENCE, ISSN 0340-255X ; 12
Keyword [en]
MEMBRANE-PROTEIN CRYSTALLIZATION; LIPIDIC CUBIC PHASES; DIAGRAM
National Category
Physical Sciences
Identifiers
URN: urn:nbn:se:miun:diva-13490DOI: 10.1007/b11618ISI: 000223033700009ISBN: 3-540-00553-6 (print)OAI: oai:DiVA.org:miun-13490DiVA: diva2:409492
Conference
15th Conference of the European-Colloid-and-Interface-Society, Sep 16-21, 2001, Coimbra, Portugal
Available from: 2011-04-08 Created: 2011-04-08 Last updated: 2014-04-23Bibliographically approved

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Persson, GerdEdlund, Håkan
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CiteExportLink to record
Permanent link

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Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
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Language
  • de-DE
  • en-GB
  • en-US
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  • nn-NO
  • nn-NB
  • sv-SE
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More languages
Output format
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  • asciidoc
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