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Enantiorecognition of Chiral Acids by Candida rugosa Lipase: Two Substrate Binding Modes Evidenced in an Organic Medium
Department of Biotechnology, Royal Institute of Technology, SE-100 44 Stockholm.
Department of Biotechnology, Royal Institute of Technology, SE-100 44 Stockholm.
Mid Sweden University, Faculty of Science, Technology and Media, Department of Natural Sciences.ORCID iD: 0000-0002-5543-2041
2001 (English)In: Applied Biocatalysis in Speciality Chemicals and Pharmaceuticals, 2001, 263-273 p.Conference paper, (Refereed)
Abstract [en]

We have identified the existence of different modes of binding the enantiomers of 2-methyl-branched carboxylic acids to a lipase active site by rational substrate engineering. Similar to hydrolysis, previously investigated, we have now evidence for differential binding modes in the Candida rugosa lipase-catalyzed esterifications in cyclohexane. The relevance of considering two different binding modes to understand lipase enantiorecognition is demonstrated by introducing bulky substituents on a chiral carboxylic acid which impose a different orientation of the substrate acyl chain in the active site of Candida rugosa lipase. With this substrate engineering approach based on molecular modeling it is thus possible to markedly alter the enantioselectivity of the lipase. Examples from hydrolysis and new results from esterifications in an organic solvent are presented and discussed.

Place, publisher, year, edition, pages
2001. 263-273 p.
Series
ACS Symposium Series, ISSN 0097-6156 ; 776
Keyword [en]
Lipase catalysis
National Category
Chemical Sciences
Identifiers
URN: urn:nbn:se:miun:diva-6042Scopus ID: 0041916056Local ID: 1866ISBN: 0-8412-3679-8 (print)OAI: oai:DiVA.org:miun-6042DiVA: diva2:31075
Available from: 2008-09-30 Created: 2008-09-30 Last updated: 2016-09-29Bibliographically approved

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