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MIGRATION OF HUMAN GRANULOCYTES IN FILTERS - EFFECTS OF GRAVITY AND MOVABLE GRADIENTS OF F-MLP
1994 (English)In: Biorheology, ISSN 0006-355X, E-ISSN 1878-5034, Vol. 31, no 6, p. 617-630Article in journal (Refereed) Published
Abstract [en]

The Boyden chamber technique for chemotaxis uses a mesh filter that constitutes a matrix for cell locomotion and, at the same time, creates a local restriction for convective fluid movements that allows the establishment of a diffusive concentration gradient of chemotactic substance in the filter. In the present study, the Boyden chamber was modified by the introduction of a filter sandwich that allowed cell migration both upwards and downwards and by the use of a fluid density gradient controlling cell buoyancy and mechanically supporting a movable chemotactic gradient, This method was used to study chemotaxis and random migration of human granulocytes under the influence of gravitational forces and movable gradients of f-MLP. The results show that gravity affected cell motion significantly during random migration but not during chemotaxis, The rate of chemotactic migration was dependent on the steepness of the spatio-temporal f-MLP gradients, A stationary spatial gradient produced less migration than a gradient that was slowly moved through the filter sandwich in a direction opposite to that of the The presence of f-MLP at constant concentration caused a minor, statistically insignificant, increase of the rate of random migration.

Place, publisher, year, edition, pages
THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB: PERGAMON-ELSEVIER SCIENCE LTD , 1994. Vol. 31, no 6, p. 617-630
Identifiers
URN: urn:nbn:se:miun:diva-11309OAI: oai:DiVA.org:miun-11309DiVA, id: diva2:298377
Available from: 2010-02-22 Created: 2010-02-22 Last updated: 2017-12-12Bibliographically approved

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Ebrahimzadeh, PR

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