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Fingerprinting metal-containing biomolecules after reductive displacement of iron by gallium and subsequent column-switched LC-ICP-MS analysis applied on siderophore.
Mid Sweden University, Faculty of Science, Technology and Media, Department of Natural Sciences.
Mid Sweden University, Faculty of Science, Technology and Media, Department of Natural Sciences.
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2004 (English)In: Analytical Chemistry, ISSN 0003-2700, E-ISSN 1520-6882, Vol. 76, no 9, p. 2618-2622Article in journal (Refereed) Published
Abstract [en]

Column-switching liquid chromatography followed by lowresolution ICPMS was evaluated as a tool for speciation analysis of metal-containing biomolecules. The strategy was applied on siderophores, strong iron chelators of low molecular weight (M-w < 1500). Prior to the LC-ICPMS analysis, reductive displacement of iron by gallium was performed using ascorbate as the reducing agent to increase the sensitivity. Different experimental conditions during the exchange reaction were tested using ferrichrysin and ferrichrome for evaluation. A reaction time of 30 min and a pH of 3.9 gave an exchange yield of 27 and 83% for ferrichrysin and ferrichrome, respectively. A gradient elution profile was also developed to separate gallium-chelated siderophores on a PGC column. Detection limits for standard solutions of ferrichrysin and ferrichrome in the low-nanomolar range were obtained by monitoring the gallium-69 isotope. The combined use of LC-ICPMS and LC-ESI-MS/MS was also evaluated as a tool to identify unknown metal complexes, here siderophores, in field soil solution samples.

Place, publisher, year, edition, pages
2004. Vol. 76, no 9, p. 2618-2622
Keywords [en]
LC-ICP-MS, siderophores, biomolecules
National Category
Chemical Sciences
Identifiers
URN: urn:nbn:se:miun:diva-2535DOI: 10.1021/ac0355000ISI: 000221205900036Local ID: 1797OAI: oai:DiVA.org:miun-2535DiVA, id: diva2:27567
Available from: 2008-09-30 Created: 2008-09-30 Last updated: 2017-12-12Bibliographically approved

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Holmström, SaraLundström, Ulla

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CiteExportLink to record
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  • apa
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