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Novel approach to the determination of structurally similar hydroxamate siderophores by column-switching capillary liquid chromatography coupled to mass spectrometry
Mid Sweden University, Faculty of Science, Technology and Media, Department of Natural Sciences.
Mid Sweden University, Faculty of Science, Technology and Media, Department of Natural Sciences.
2003 (English)In: Journal of Chromatography A, ISSN 0021-9673, E-ISSN 1873-3778, Vol. 1020, no 1, 91-97 p.Article in journal (Refereed) Published
Abstract [en]

In this study a new approach to determine three different siderophores (ferrichrome, ferrichrysin, ferricrocin) in natural soil solutions as well as in cultures of fungi is presented. The method includes enrichment of the analytes on a short pre-column, packed with C18 material, and subsequent highly selective separation of the analytes on a capillary porous graphitic carbon (PGC) column. In contrast to normal C18 packing materials, porous graphitic carbon offers chromatographic resolution between the three very similar analytes. The selectivity of the method is enhanced even further by the electrospray ionization (ESI) mass spectrometric detection. The combination of a short pre-column and a packed capillary separation column results in a method with high sensitivity. Reported detection limits, defined as the concentration giving the signal-to-noise ratio 3:1, is 27.7 pM for ferrichrome, 46.1 pM for ferricrocin and 37.4 pM for ferrichrysin.

Place, publisher, year, edition, pages
2003. Vol. 1020, no 1, 91-97 p.
Keyword [en]
Porous graphitic carbon, Column-switching, Soil, Siderophores, Ferrichrome, Ferricrocin, Ferrichrysin, Hydroxamates
National Category
Chemical Sciences
Identifiers
URN: urn:nbn:se:miun:diva-572DOI: 10.1016/S0021-9673(03)01236-6ISI: 000186503900010Scopus ID: 1642350898Local ID: 1802 1106OAI: oai:DiVA.org:miun-572DiVA: diva2:25202
Note
19th Montreux Symposium on Liquid Chromatograph, Nov 06-08-08, 2002, Montreux, SwitzerlandAvailable from: 2008-09-23 Created: 2008-09-23 Last updated: 2011-07-26Bibliographically approved
In thesis
1. Organic acids and siderophores in forest soil solution
Open this publication in new window or tab >>Organic acids and siderophores in forest soil solution
2003 (English)Doctoral thesis, comprehensive summary (Other academic)
Place, publisher, year, edition, pages
Sundsvall: Mittuniversitetet, 2003
Series
Mid Sweden University doctoral thesis, ISSN 1652-893X ; 0
Keyword
acidification, aluminium, ash treatment, canopy tree, column switching, coniferous, DOC, HPLC-MC, hydroxamate, liming, low molecular weight, organic acids, podzol, porous graphitic carbon, siderophore, soil solution
National Category
Chemical Sciences
Identifiers
urn:nbn:se:miun:diva-8904 (URN)91-87908-76-X (ISBN)
Public defence
2003-12-05, Sundsvall, 13:00 (English)
Available from: 2009-05-07 Created: 2009-05-07 Last updated: 2017-03-16Bibliographically approved

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Citation style
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