River Ljungan in Sweden has suffered from crayfish plague (Aphanomyces astacii), resulting in the extinction of noble crayfish (Astacus astacus). At the same time, P2X industries such as hydrogen gas production is developing in the region. The Swedish Hydrogen and Crayfish research project explores how the residue products from the production of hydrogen gas; oxygen, and heat, can be utilized to disinfect crayfish plague and facilitate the growth and survival of crayfish. The temperature of process water varies between 48 and 73°C and coulddisinfect water from crayfish plague as spores of crayfish plague die from a short exposure to 60°C. Ozone disinfection and UV light are common techniques to neutralize microorganisms in drinking water. It is unclear how these techniques affect the survival of crayfish plague spores. This presentation outlines a study, in which solutions for direct or indirect water supply from hydrogen production to crayfish farms will be proposed. The goal is to ensure that crayfish farming can be freed from crayfish plague and has a temperature that favors the survival and growth of the crayfish. Laboratory experiments will test different methods to disinfect crayfish plague. The presence of crayfish plague and the survival of crayfish in the treated water will then be investigated. Crayfish plague occurrence will be measured with a real-time PCR Thermocycler before and after treatment of water. The genotype of the crayfish plague will be analyzed with TaqMan MGB real-time PCR. We will present the experimental design and would appreciate your input.