An increasing consciousness toward the reduction of the environmental impact producedby plastic waste, has driven research and industry to focus on using biodegradable materials. Among those biodegradable materials, polysaccharides are regarded to have great potential in packaging applications, because of their outstanding performance with biocompatibility, biodegradability, and good processability. Naturally, most of the polysaccharides do not exhibit desired barrier properties, due to their hydrophilic nature, which hinder their application in packaging field. The incorporation of reinforcing structures such as cellulosenanocrystals (CNCs) in these polysaccharides is promising approach to produce new materials for food packaging application with specific properties and high performances. CNCs have been mostly used as reinforcing phase in matrices for a variety of materials, and thus given to their outstanding properties such as exceptional mechanical strength, biocompatibility, broadchemical modification, and large surface area. The aim of this study is the development of biodegradable films based on polysaccharides and CNCs from different ressources using agreen method such as the evaporation casting method and focusing on the final produced material properties related to food packaging applications (mechanical properties, thermal properties, antibacterial properties, optical transparency and resistance to water vapor and oxygen transmission).