miun.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Spectral properties and chromophore rotation of phytochrome bound to substituted Sepharose
Dept of Plant Physiology, Univ. of Lund.
1986 (English)In: Physiologia Plantarum: An International Journal for Plant Biology, ISSN 0031-9317, Vol. 66, 185-190 p.Article in journal (Refereed) Published
Abstract [en]

Brushite purified phytochrome from Avena sativa L. cv. Sol II was bound to phenyl Sepharose, octyl Sepharose, CNBr-activated Sepharose and to anti-phytochrome immunoglobulins immobilized on Sepharose. The spectral properties of phytochrome bound to anti-phytochrome immunoglobulins and to phenyl Sepharose were similar to phytochrome in solution. Phytochrome bound to CNBr-activated Sepharose or to octyl Sepharose showed reduced Pfr formation after red irradiation. The reversal to Pr with far-red light was only partial but a further increase at 667 nm took place slowly in the dark. A peak at 657 nm was seen in the difference spectrum between CNBr-activated Sepharose-bound phytochrome kept in darkness and the identical sample immediately after a far-red irradiation.

The change in linear dichroism at 660 nm and 730 nm, induced by plane polarized red or far-red light, was measured. It was computed that the long-wavelength transition moment of phytochrome had an average rotation angle of 31.5° or 180°–31.5°. The substrate used for immobilization had a limited effect on the rotation angle. Phytochrome immobilized on CNBr-activated Sepharose gave an angle of 27.8° and phytochrome immobilized on phenyl Sepharose gave an angle of 32.6°.

Place, publisher, year, edition, pages
1986. Vol. 66, 185-190 p.
Keyword [en]
Avena sativa • linear dichroism • phytochrome intermediates
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:miun:diva-7552DOI: 10.1111/j.1399-3054.1986.tb02406.xOAI: oai:DiVA.org:miun-7552DiVA: diva2:127702
Available from: 2008-12-10 Created: 2008-12-09 Last updated: 2009-11-18Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Ekelund, Nils
In the same journal
Physiologia Plantarum: An International Journal for Plant Biology
Biological Sciences

Search outside of DiVA

GoogleGoogle Scholar

Altmetric score

Total: 45 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf