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Influence of chlorophyll concentrations on the reliability of measurements with an oxygen electrode when studying photosynthetic efficiency in bioassays using microalgae
Mid Sweden University, Faculty of Science, Technology and Media, Department of Natural Sciences.
Mid Sweden University, Faculty of Science, Technology and Media, Department of Natural Sciences.
2000 (English)In: Journal of Applied Phycology, ISSN 0921-8971, E-ISSN 1573-5176, Vol. 12, no 1, 63-67 p.Article in journal (Refereed) Published
Abstract [en]

Euglena gracilis and Chlamydomonas reinhardtii were used as model organisms to establish the best conditions for studying photosynthetic efficiency using the Light Pipetteexperimental system, which enables sensitive detection of changes in oxygen evolution/consumption and an easy collection and digitalisation of data. Chlorophyll concentrations of 0.005, 0.025, 0.050 and 0.075 mg mL-1 were investigated using different light regimes. Cultures of E. gracilis at the same chlorophyll concentration absorbed more light (measured at 580 ╬╝mol m-2 s-1) than those of C. reinhardtii. Cell density had a considerable effect on the reliability of measurements. Chlorophyll concentrations between 0.025 mg(Chl) ml-1 and 0.050 mg(Chl) ml-1 can be recommended when applying the Light Pipette system in bioassays using microalgae.

Place, publisher, year, edition, pages
2000. Vol. 12, no 1, 63-67 p.
Keyword [en]
microalgae, Euglena gracilis; Chlamydomonas reinhardtii; photosynthesis; oxygen electrode; Light Pipette; bioassay; chlorophyll concentration; algae
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:miun:diva-7525DOI: 10.1023/A:1008189230953ISI: 000088076100008Scopus ID: 2-s2.0-0033902771OAI: oai:DiVA.org:miun-7525DiVA: diva2:127672
Available from: 2008-12-09 Created: 2008-12-09 Last updated: 2016-10-10Bibliographically approved

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CiteExportLink to record
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Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
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More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
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  • Other locale
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Output format
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  • asciidoc
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