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Megahertz serial crystallography
DESY, Hamburg, Germany; Univ Hamburg, Hamburg, Germany.
DESY, Hamburg, Germany.
European XFEL GmbH, Schenefeld, Germany.
Univ Hamburg, Hamburg, Germany; Integrated Biol Infrastruct Life Sci Facil Europe, Schenefeld, Germany.
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2018 (English)In: Nature Communications, ISSN 2041-1723, E-ISSN 2041-1723, Vol. 9, article id 4025Article in journal (Refereed) Published
Abstract [en]

The new European X-ray Free-Electron Laser is the first X-ray free-electron laser capable of delivering X-ray pulses with a megahertz inter-pulse spacing, more than four orders of magnitude higher than previously possible. However, to date, it has been unclear whether it would indeed be possible to measure high-quality diffraction data at megahertz pulse repetition rates. Here, we show that high-quality structures can indeed be obtained using currently available operating conditions at the European XFEL. We present two complete data sets, one from the well-known model system lysozyme and the other from a so far unknown complex of a beta-lactamase from K. pneumoniae involved in antibiotic resistance. This result opens up megahertz serial femtosecond crystallography (SFX) as a tool for reliable structure determination, substrate screening and the efficient measurement of the evolution and dynamics of molecular structures using megahertz repetition rate pulses available at this new class of X-ray laser source.

Place, publisher, year, edition, pages
2018. Vol. 9, article id 4025
National Category
Electrical Engineering, Electronic Engineering, Information Engineering
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URN: urn:nbn:se:miun:diva-34889DOI: 10.1038/s41467-018-06156-7ISI: 000446016400003PubMedID: 30279492Scopus ID: 2-s2.0-85054197272OAI: oai:DiVA.org:miun-34889DiVA, id: diva2:1264092
Available from: 2018-11-19 Created: 2018-11-19 Last updated: 2019-03-22Bibliographically approved

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Graafsma, Heinz

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