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Titanium ions form particles that activate and execute interleukin-1β release from lipopolysaccharide-primed macrophages
Prosthetic Dentistry Department of Odontology Faculty of Medicine, Umeå University, Umeå.
Department of Integrative Medical Biology Faculty of Medicine, Umeå University, Umeå.
Section of Oral Microbiology and Immunology Institute of Oral Biology Center of Dental Medicine, University of Zürich, Zürich, Switzerland.
Mid Sweden University, Faculty of Science, Technology and Media, Department of Natural Sciences.ORCID iD: 0000-0002-3646-294X
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2016 (English)In: Journal of Periodontal Research, ISSN 0022-3484, E-ISSN 1600-0765Article in journal (Refereed) Published
Abstract [en]

Background and Objective: Peri-implantitis is a destructive inflammatory process characterized by destruction of the implant-supporting bone. Inflammasomes are large intracellular multiprotein complexes that play a central role in innate immunity by activating the release of proinflammatory cytokines. Although inflammasome activation has previously been linked to periodontal inflammation, there is still no information on a potential association with peri-implantitis. The aim of this study was to examine cytotoxic and proinflammatory effects, including inflammasome activation, of metals used in dental implants, in an in vitro model, as well as from clinical tissue samples. Material and methods: Human macrophages were exposed to different metals [titanium (Ti), cobalt, chromium and molybdenum] in a cell-culture assay. Cytotoxicity was determined using the neutral red uptake assay. Cytokine secretion was quantified using an ELISA, and the expression of genes of various inflammasome components was analysed using quantitative PCR. In addition, the concentrations of interleukin-1β (IL-1β) and Ti in mucosal tissue samples taken in the vicinity of dental implants were determined using ELISA and inductively coupled plasma mass spectrometry, respectively. Results: Ti ions in physiological solutions stimulated inflammasome activation in human macrophages and consequently IL-1β release. This effect was further enhanced by macrophages that have been exposed to lipopolysaccharides. The proinflammatory activation caused by Ti ions disappeared after filtration (0.22 μm), which indicates an effect of particles. Ti ions alone did not stimulate transcription of the inflammasome components. The Ti levels of tissue samples obtained in the vicinity of Ti implants were sufficiently high (≥ 40 μm) to stimulate secretion of IL-1β from human macrophages in vitro. Conclusion: Ti ions form particles that act as secondary stimuli for a proinflammatory reaction.

Place, publisher, year, edition, pages
2016.
Keyword [en]
Caspase-1, Inflammation, Interleukin-1β, Macrophage, Peri-implantitis, Titanium
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:miun:diva-29603DOI: 10.1111/jre.12364ISI: 000393165200003PubMedID: 26987886ScopusID: 2-s2.0-84961176243OAI: oai:DiVA.org:miun-29603DiVA: diva2:1056898
Available from: 2016-12-15 Created: 2016-12-15 Last updated: 2017-03-06Bibliographically approved

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