Skeletal muscle glycogen content and particle size of distinct subcellular localizations in the recovery period after a high-level soccer matchShow others and affiliations
2012 (English)In: European Journal of Applied Physiology, ISSN 1439-6319, E-ISSN 1439-6327, Vol. 112, no 10, p. 3559-3567Article in journal (Refereed) Published
Abstract [en]
Whole muscle glycogen levels remain low for a prolonged period following a soccer match. The present study was conducted to investigate how this relates to glycogen content and particle size in distinct subcellular localizations. Seven high-level male soccer players had a vastus lateralis muscle biopsy collected immediately after and 24, 48, 72 and 120 h after a competitive soccer match. Transmission electron microscopy was used to estimate the subcellular distribution of glycogen and individual particle size. During the first day of recovery, glycogen content increased by ~60% in all subcellular localizations, but during the subsequent second day of recovery glycogen content located within the myofibrils (Intramyofibrillar glycogen, a minor deposition constituting 10–15% of total glycogen) did not increase further compared with an increase in subsarcolemmal glycogen (−7 vs. +25%, respectively, P = 0.047). Conversely, from the second to the fifth day of recovery, glycogen content increased (53%) within the myofibrils compared to no change in subsarcolemmal or intermyofibrillar glycogen (P < 0.005). Independent of location, increment in particle size preceded increment in number of particles. Intriguingly, average particle size decreased; however, in the period from 3 to 5 days after the match. These findings suggest that glycogen storage in skeletal muscle is influenced by subcellular localization-specific mechanisms, which account for an increase in number of glycogen particles located within the myofibrils in the period from 2 to 5 days after the soccer match.
Place, publisher, year, edition, pages
2012. Vol. 112, no 10, p. 3559-3567
Keywords [en]
Carbohydrate metabolism; Glycogen storage disease; Muscle contraction; Muscle fatigue; Physical exertion; Skeletal muscle fibres
National Category
Sport and Fitness Sciences
Identifiers
URN: urn:nbn:se:miun:diva-18330DOI: 10.1007/s00421-012-2341-9ISI: 000308646000012Scopus ID: 84868582210OAI: oai:DiVA.org:miun-18330DiVA, id: diva2:600086
2013-01-232013-01-232017-12-06Bibliographically approved