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Extraction of Antioxidants from Spruce (Picea abies) Bark using Eco-friendly Solvents
Dept Phys & Analyt Chem, Uppsala Univ, POB 599, SE-75124 Uppsala.
Mittuniversitetet, Fakulteten för naturvetenskap, teknik och medier, Institutionen för tillämpad naturvetenskap och design.
Dept Biochem & Organ Chem, Uppsala Univ, SE-75123 Uppsala.
Mittuniversitetet, Fakulteten för naturvetenskap, teknik och medier, Institutionen för tillämpad naturvetenskap och design.
Vise andre og tillknytning
2012 (engelsk)Inngår i: Phytochemical Analysis, ISSN 0958-0344, E-ISSN 1099-1565, Vol. 23, nr 1, s. 1-11Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Introduction - Antioxidants are known to avert oxidation processes and they are found in trees and other plant materials. Tree bark is a major waste product from paper pulp industries; hence it is worthwhile to develop an extraction technique to extract the antioxidants. Objective - To develop a fast and environmentally sustainable extraction technique for the extraction of antioxidants from bark of spruce (Picea abies) and also to identify the extracted antioxidants that are abundant in spruce bark. Methodology - A screening experiment that involved three different techniques was conducted to determine the best technique to extract antioxidants. The antioxidant capacity of the extracts was determined with DPPH (2,2-diphenyl-1-picrylhydrazyl) assay. Pressurised fluid extraction (PFE) turned out to be the best technique and a response surface design was therefore utilised to optimise PFE. Furthermore, NMR and HPLC-DAD-MS/MS were applied to identify the extracted antioxidants. Results - PFE using water and ethanol as solvent at 160 and 180°C, respectively, gave extracts of the highest antioxidant capacity. Stilbene glucosides such as isorhapontin, piceid and astringin were identified in the extracts. Conclusion - The study has shown that PFE is a fast and environmentally sustainable technique, using water and ethanol as solvent for the extraction of antioxidants from spruce bark. Copyright © 2011 John Wiley and Sons, Ltd. This paper includes four tables in which the extracted solid yield and the antioxidant capacity of spruce bark extracts were presented for the different extraction techniques, as well as the response surface coefficients for the optimised PFE were presented. © 2011 John Wiley and Sons, Ltd..

sted, utgiver, år, opplag, sider
2012. Vol. 23, nr 1, s. 1-11
Emneord [en]
Accelerated solvent extraction, Antioxidant, DPPH, Ethanol, Picea abies, Pressurised fluid extraction, Stilbene glucosides, Supercritical fluid extraction, Water
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Identifikatorer
URN: urn:nbn:se:miun:diva-14214DOI: 10.1002/pca.1316ISI: 000298260100001Scopus ID: 2-s2.0-82955198459OAI: oai:DiVA.org:miun-14214DiVA, id: diva2:431445
Tilgjengelig fra: 2011-07-19 Laget: 2011-07-19 Sist oppdatert: 2017-12-08bibliografisk kontrollert
Inngår i avhandling
1. Naturally occurring phenols with antioxidant, antifungal and anti-browsing activity
Åpne denne publikasjonen i ny fane eller vindu >>Naturally occurring phenols with antioxidant, antifungal and anti-browsing activity
2014 (engelsk)Doktoravhandling, med artikler (Annet vitenskapelig)
sted, utgiver, år, opplag, sider
Sundsvall: Mid Sweden University, 2014. s. 68
Serie
Mid Sweden University doctoral thesis, ISSN 1652-893X ; 198
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urn:nbn:se:miun:diva-24028 (URN)978-91-87557-77-4 (ISBN)
Disputas
2014-09-26, 11:00
Veileder
Tilgjengelig fra: 2015-01-02 Laget: 2015-01-02 Sist oppdatert: 2015-03-13bibliografisk kontrollert

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