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Role of glycogen availability in sarcoplasmic reticulum Ca(2+) kinetics in human skeletal muscle
University of Southern Denmark, Odense, Denmark.
University of Southern Denmark, Odense, Denmark.
Copenhagen Muscle Research Centre, Copenhagen, Denmark.
Mid Sweden University, Faculty of Human Sciences, Department of Health Sciences. (Swedish Winter Spoirts Research Centre)ORCID iD: 0000-0002-3814-6246
2011 (English)In: Journal of Physiology, ISSN 0022-3751, E-ISSN 1469-7793, Vol. 589, no 3, p. 711-725Article in journal (Refereed) Published
Abstract [en]

Little is known about the precise mechanism that relates skeletal muscle glycogen to muscle fatigue. The aim of the present study was to examine the effect of glycogen on sarcoplasmic reticulum (SR) function in the arm and leg muscles of elite cross-country skiers (n = 10, (V) over dot(O2 max) 72 +/- 2 ml kg(-1) min(-1)) before, immediately after, and 4 h and 22 h after a fatiguing 1 h ski race. During the first 4 h recovery, skiers received either water or carbohydrate (CHO) and thereafter all received CHO-enriched food. Immediately after the race, arm glycogen was reduced to 31 +/- 4% and SR Ca2+ release rate decreased to 85 +/- 2% of initial levels. Glycogen noticeably recovered after 4 h recovery with CHO (59 +/- 5% initial) and the SR Ca2+ release rate returned to pre-exercise levels. However, in the absence of CHO during the first 4 h recovery, glycogen and the SR Ca2+ release rate remained unchanged (29 +/- 2% and 77 +/- 8%, respectively), with both parameters becoming normal after the remaining 18 h recovery with CHO. Leg muscle glycogen decreased to a lesser extent (71 +/- 10% initial), with no effects on the SR Ca2+ release rate. Interestingly, transmission electron microscopy (TEM) analysis revealed that the specific pool of intramyofibrillar glycogen, representing 10-15% of total glycogen, was highly significantly correlated with the SR Ca2+ release rate. These observations strongly indicate that low glycogen and especially intramyofibrillar glycogen, as suggested by TEM, modulate the SR Ca2+ release rate in highly trained subjects. Thus, low glycogen during exercise may contribute to fatigue by causing a decreased SR Ca2+ release rate.

Place, publisher, year, edition, pages
2011. Vol. 589, no 3, p. 711-725
Keywords [en]
PROLONGED EXERCISE; INTRACELLULAR CALCIUM; SUBCELLULAR GLYCOGEN; CANE TOAD; FATIGUE; RELEASE; FIBERS; RAT; LOCALIZATION; PHOSPHORYLASE
National Category
Physiology Nutrition and Dietetics Sport and Fitness Sciences
Identifiers
URN: urn:nbn:se:miun:diva-11883DOI: 10.1113/jphysiol.2010.195982ISI: 000286744100024Scopus ID: 2-s2.0-79251576463OAI: oai:DiVA.org:miun-11883DiVA, id: diva2:332021
Available from: 2010-07-29 Created: 2010-07-29 Last updated: 2018-01-12Bibliographically approved

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Holmberg, Hans-Christer

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