miun.sePublikasjoner
Endre søk
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
On the importance of controlling film architecture in detecting prostate specific antigen
Federal University of São Carlos, Center for Sciences and Technology for Sustainability, Sorocaba, SP, Brazil.
Federal University of São Carlos, Center for Sciences and Technology for Sustainability, Sorocaba, SP, Brazil.
University of São Paulo, São Carlos, SP, Brazil.
Mittuniversitetet, Fakulteten för naturvetenskap, teknik och medier, Avdelningen för naturvetenskap.
Vise andre og tillknytning
2018 (engelsk)Inngår i: Applied Surface Science, ISSN 0169-4332, E-ISSN 1873-5584, Vol. 434, s. 1175-1182Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Immunosensors made with nanostructured films are promising for detecting cancer biomarkers, even at early stages of the disease, but this requires control of film architecture to preserve the biological activity of immobilized antibodies. In this study, we used electrochemical impedance spectroscopy (EIS) to detect Prostate Specific Antigen (PSA) with immunosensors produced with layer-by-layer (LbL) films containing anti-PSA antibodies in two distinct film architectures. The antibodies were either adsorbed from solutions in which they were free, or from solutions where they were incorporated into liposomes of dipalmitoyl phosphatidyl glycerol (DPPG). Incorporation into DPPG liposomes was confirmed with surface plasmon resonance experiments, while the importance of electrostatic interactions on the electrical response was highlighted using the Finite Difference Time-Domain Method (FDTD). The sensitivity of both architectures was sufficient to detect the threshold value to diagnose prostate cancer (ca. 4 ng mL−1). In contrast to expectation, the sensor with the antibodies incorporated into DPPG liposomes had lower sensitivity, though the range of concentrations amenable to detection increased, according to the fitting of the EIS data using the Langmuir-Freundlich adsorption model. The performance of the two film architectures was compared qualitatively by plotting the data with a multidimensional projection technique, which constitutes a generic approach for optimizing immunosensors and other types of sensors. 

sted, utgiver, år, opplag, sider
2018. Vol. 434, s. 1175-1182
Emneord [en]
Electrochemical impedance spectroscopy, Finite difference time-domain method, Immunosensor, Layer-by-layer, Liposome
HSV kategori
Identifikatorer
URN: urn:nbn:se:miun:diva-32286DOI: 10.1016/j.apsusc.2017.10.122ISI: 000419116600135Scopus ID: 2-s2.0-85033572501OAI: oai:DiVA.org:miun-32286DiVA, id: diva2:1163209
Tilgjengelig fra: 2017-12-06 Laget: 2017-12-06 Sist oppdatert: 2019-08-06bibliografisk kontrollert

Open Access i DiVA

Fulltekst mangler i DiVA

Andre lenker

Forlagets fulltekstScopus

Personposter BETA

Volpati, Diogo

Søk i DiVA

Av forfatter/redaktør
Volpati, Diogo
Av organisasjonen
I samme tidsskrift
Applied Surface Science

Søk utenfor DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetric

doi
urn-nbn
Totalt: 19 treff
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf