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Viklund, Lina
Publications (2 of 2) Show all publications
Rahmani, R., Wallin, E., Viklund, L., Schroeder, M. & Hedenström, E. (2019). Identification and Field Assay of Two Aggregation Pheromone Components Emitted by Males of the Bark Beetle Polygraphus punctifrons (Coleoptera: Curculionidae). Journal of Chemical Ecology, 45(4), 356-365
Open this publication in new window or tab >>Identification and Field Assay of Two Aggregation Pheromone Components Emitted by Males of the Bark Beetle Polygraphus punctifrons (Coleoptera: Curculionidae)
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2019 (English)In: Journal of Chemical Ecology, ISSN 0098-0331, E-ISSN 1573-1561, Vol. 45, no 4, p. 356-365Article in journal (Refereed) Published
Abstract [en]

The bark beetle Polygraphus punctifrons (Coleoptera: Curculionidae) is a species that feeds on Norway spruce (Picea abies) and is found in the Northern parts of Europe and Russia. The release of volatile organic compounds (VOCs) produced by males and females of P. punctifrons when the beetles bore into spruce stem sections in a laboratory environment was studied using solid phase microextraction (SPME). The sampled VOCs emitted by boring beetles were analysed by gas chromatography and mass spectrometry (GCMS). (+)-2-[(1R,2S)-1-Methyl-2-(prop-1-en-2-yl)cyclobutyl]ethanol [(+)-(1R,2S)-grandisol] and (−)-(R)-1-isopropyl-4-methyl-3-cyclohexen-1-ol [(−)-(R)-terpinen-4-ol] were identified to be male specific volatiles. The identity of the compounds was confirmed by comparison with synthetic samples. Field trials with synthetic compounds in Sweden showed that racemic grandisol per se was strongly attractive for both males and females, while (−)-(R)-terpinen-4-ol was not. Further, when adding (−)-(R)-terpinen-4-ol to rac-grandisol, a synergistic effect was observed as the trap catch of P. punctifrons was fourfold. (−)-(R)-Terpinen-4-ol by its own did not attract P. punctifrons but Polygraphus poligraphus, and the latter was also attracted to traps baited with a 10:90 mixture of the two compounds. Thus, we have identified (+)-(1R,2S)-grandisol as a main component and (−)-(R)-terpinen-4-ol as a minor component of the aggregation pheromone of P. punctifrons. This opens future possibilities to monitor and, if necessary, manage populations of P. punctifrons. 

Keywords
(+)-(1R, 2S)-Grandisol, (−)-(R)-Terpinen-4-ol, Enantiomeric separation, Picea abies, Preparative fraction collection, SPME
National Category
Chemical Engineering
Identifiers
urn:nbn:se:miun:diva-35836 (URN)10.1007/s10886-019-01056-6 (DOI)000467745400003 ()30796678 (PubMedID)2-s2.0-85061967619 (Scopus ID)
Available from: 2019-03-20 Created: 2019-03-20 Last updated: 2019-08-14Bibliographically approved
Viklund, L., Rahmani, R., Bång, J., Schroeder, M. & Hedenström, E. (2019). Optimizing the attractiveness of pheromone baits used for trapping the four-eyed spruce bark beetle Polygraphus poligraphus. Journal of applied entomology, 143(7), 721-730
Open this publication in new window or tab >>Optimizing the attractiveness of pheromone baits used for trapping the four-eyed spruce bark beetle Polygraphus poligraphus
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2019 (English)In: Journal of applied entomology, ISSN 0931-2048, E-ISSN 1439-0418, Vol. 143, no 7, p. 721-730Article in journal (Refereed) Published
Abstract [en]

Bark beetles have caused extensive damage to forests in central Sweden during the past decade, and the four-eyed spruce bark beetle, Polygraphus poligraphus, seems to be involved. However, its role in these bark beetle outbreaks is still not clear. The purpose of this study was to develop an efficient pheromone bait for P. poligraphus, which would make it possible to study the species more carefully and thereby contribute to protect exposed forests in an environmentally friendly way. Three field studies were conducted in 2015, 2016 and 2018 in Medelpad, county of Västernorrland, Sweden. The pheromone of P. poligraphus, (−)-terpinen-4-ol, was tested at different release rates and in different enantiomeric purities, to find the most attractive formulation for the beetles. It was also tested in combination with racemic frontalin, a compound which has previously been shown to produce a synergistic effect together with (−)-terpinen-4-ol of low enantiomeric purity; 52% ee. Other compounds, chosen based on responses from electroantennographic studies, were also tested in an attempt to find additional attractants and repellents for P. poligraphus. The most attractive treatment tested was enantiomerically pure (−)-terpinen-4-ol (99% ee). When the enantiomeric purity was lower (50% ee), the trap catches was lowered to levels comparable to the catches for unbaited control traps. A strong synergistic effect with frontalin was observed for (−)-terpinen-4-ol of low enantiomeric purity (50% ee) but not for the enantiomerically pure compound (99% ee). The release rate of (−)-terpinen-4-ol (99% ee) was shown to be an important factor. For the combination of frontalin and (−)-terpinen-4-ol (50% ee), the attraction seemed strongest when (−)-terpinen-4-ol was released at a higher rate than frontalin. An interesting and novel result was that a repellent compound, α-terpineol, was identified in our studies. Our results from field studies and electroantennography recordings also indicate that (+)-terpinen-4-ol is a repellent for P. poligraphus.

Keywords
enantiomers, field study, frontalin, pheromone, synergism, terpinen‐4‐ol
National Category
Ecology
Identifiers
urn:nbn:se:miun:diva-36659 (URN)10.1111/jen.12641 (DOI)000475465300003 ()2-s2.0-85064480153 (Scopus ID)
Available from: 2019-07-08 Created: 2019-07-08 Last updated: 2019-10-16Bibliographically approved
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