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Gylle, A. Maria
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Publications (10 of 15) Show all publications
Gylle, A. M., Nygård, C. A., Svan, C. I., Pocock, T. & Ekelund, N. G. A. (2013). Photosynthesis in relation to D1, PsaA and Rubisco in marine and brackish water ecotypes of Fucus vesiculosus and Fucus radicans (Phaeophyceae). Hydrobiologia, 700(1), 109-119
Open this publication in new window or tab >>Photosynthesis in relation to D1, PsaA and Rubisco in marine and brackish water ecotypes of Fucus vesiculosus and Fucus radicans (Phaeophyceae)
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2013 (English)In: Hydrobiologia, ISSN 0018-8158, E-ISSN 1573-5117, Vol. 700, no 1, p. 109-119Article in journal (Refereed) Published
Abstract [en]

The aim of this study was to investigate photosynthetic differences between the marine, Norwegian Sea ecotype and the brackish, Bothnian Sea ecotype of F. vesiculosus and F. radicans and to see whether photosynthetic differences could be connected with the relative amounts of D1 protein (PSII), PsaA (PSI) protein and/or Rubisco. For this purpose, we tested if a higher photosynthetic maximum (P (max)) in the Atlantic Ocean ecotype of F. vesiculosus relative to the Baltic Sea ecotype, and an increase of the P (max) in Baltic Sea ecotype of F. vesiculosus at higher salinity, could be due to an increase in the relative amounts of Rubisco. The proteins have been evaluated on a relative basis. Immunoblot signals showed that the amount of Rubisco was higher in both ecotypes of F. vesiculosus than in F. radicans, but no differences could be detected between the two ecotypes of F. vesiculosus. The results suggest an uneven photosystem protein stoichiometry in Fucus, with more of the PSI protein PsaA relative to the PSII protein D1. The difference in P (max) between the two ecotypes of F. vesiculosus might be related to the difficulties for the algae to adapt to the environment in Bothnian Sea.

Keywords
D1; Fucus radicans; Fucus vesiculosus; Photosynthetic maximum capacity (P-max); PsaA; Rubisco
National Category
Botany
Identifiers
urn:nbn:se:miun:diva-18289 (URN)10.1007/s10750-012-1231-9 (DOI)000312073300010 ()2-s2.0-84870670687 (Scopus ID)
Available from: 2013-01-16 Created: 2013-01-16 Last updated: 2017-12-06Bibliographically approved
Svahn, C., Gylle, M. & Ekelund, N. G. A. (2012). Photosynthetic activity in marine and brackish water strains of Fucus vesiculosus and Fucus radicans (Phaeophyceae) at different light qualities. Photochemistry and Photobiology, 88(6), 1455-1460
Open this publication in new window or tab >>Photosynthetic activity in marine and brackish water strains of Fucus vesiculosus and Fucus radicans (Phaeophyceae) at different light qualities
2012 (English)In: Photochemistry and Photobiology, ISSN 0031-8655, E-ISSN 1751-1097, Vol. 88, no 6, p. 1455-1460Article in journal (Refereed) Published
Abstract [en]

This study investigates the effects of different light qualities on the photosynthetic capacity of the brown algae Fucus vesiculosus, from the Norwegian Sea, and Fucus radicans and F. vesiculosus, from the Bothnian Sea. The electron transport rates (ETR) obtained for F. vesiculosus from the Norwegian Sea showed significantly higher levels of light saturation compared with both species of algae from the Bothnian Sea. The maximum of ETR values for the Norwegian Sea strain showed no significant changes due to varying light quality compared with the initial values. For F. vesiculosus, from the Bothnian Sea, treatment with blue light showed an effect after 1 week of 30 and 90 μmol photons m -2 s -1 (P < 0.01), and for F. radicans from the Bothnian Sea, at the irradiance of 90 μmol photons m -2 s -1 and 1 week (P < 0.01). After 1 week in the Bothnian Sea species and after 2 weeks in F. vesiculosus from the Norwegian Sea, the photosynthetic efficiency (α) was significantly higher regardless of light quality and irradiance compared with the initial values. Variation in light quality and irradiance had minor effects on the F v:F m values of the three algal strains studied. © 2012 Wiley Periodicals, Inc. Photochemistry and Photobiology © 2012 The American Society of Photobiology.

Keywords
algae; Fucus; Fucus vesiculosus; Phaeophyceae
National Category
Biological Sciences
Identifiers
urn:nbn:se:miun:diva-17373 (URN)10.1111/j.1751-1097.2012.01187.x (DOI)000310563300016 ()2-s2.0-84868203504 (Scopus ID)
Note

CODEN: PHCBA

Available from: 2012-11-26 Created: 2012-11-19 Last updated: 2017-12-07Bibliographically approved
Gylle, A. M., Rantamäki, S., Ekelund, N. G. .. & Tyystjärvi, E. (2011). Fluorescence emission spectra of marine and brackish-water ecotypes of Fucus vesiculosus and Fucus radicans (Phaeophyceae) reveal differences in light-harvesting apparatus: emission spectra in Fucus. Journal of Phycology, 47(1), 98-105
Open this publication in new window or tab >>Fluorescence emission spectra of marine and brackish-water ecotypes of Fucus vesiculosus and Fucus radicans (Phaeophyceae) reveal differences in light-harvesting apparatus: emission spectra in Fucus
2011 (English)In: Journal of Phycology, ISSN 0022-3646, E-ISSN 1529-8817, Vol. 47, no 1, p. 98-105Article in journal (Refereed) Published
Abstract [en]

The Bothnian Sea in the northerly part of the Baltic Sea is a geologically recent brackish-water environment, and rapid speciation is occurring in the algal community of the Bothnian Sea. We measured low-temperature fluorescence emission spectra from the Bothnian Sea and the Norwegian Sea ecotypes of Fucus vesiculosus L., a marine macroalga widespread in the Bothnian Sea. Powdered, frozen thallus was used to obtain undistorted emission spectra. The spectra were compared with spectra measured from the newly identified species Fucus radicans L.Bergström et L. Kautsky, which is a close relative of F. vesiculosusand endemic to the Bothnian Sea. The spectrum of variable fluorescence was used to identify fluorescence peaks originating in PSI and PSII in this chl c–containing alga. The spectra revealed much higher PSII emission, compared to PSI emission, in the Bothnian Sea ecotype of F. vesiculosus than in F. radicans or in the Norwegian Sea ecotype of F. vesiculosus. The results suggest that more lightharvesting chl a ⁄ c proteins serve PSII in the Bothnian Sea ecotype of F. vesiculosusthan in the two other algal strains. Treatment of the Bothnian Sea ecotype of F. vesiculosusin high salinity (10, 20, and 35 practical salinity units) for 1 week did not lead to spectral changes, indicating that the measured features of the Bothnian Sea F. vesiculosus are stable and not simply a direct result of exposure to low salinity.

Keywords
Brown algae, chlorophyll a/c proteins, 77 K emission, fluorescence spectrum, Fucus vesiculosus, Fucus radicans, light-harvesting, photosystem
National Category
Biological Sciences
Identifiers
urn:nbn:se:miun:diva-10667 (URN)10.1111/j.1529-8817.2010.00928.x (DOI)000287492700011 ()2-s2.0-79951764727 (Scopus ID)
Available from: 2010-01-10 Created: 2009-12-14 Last updated: 2017-12-12Bibliographically approved
Gylle, A. M. (2011). Physiological adaptations in two ecotypes of Fucus vesiculosus and in Fucus radicans with focus on salinity. (Doctoral dissertation). Sundsvall: Kopieringen Mid Sweden University
Open this publication in new window or tab >>Physiological adaptations in two ecotypes of Fucus vesiculosus and in Fucus radicans with focus on salinity
2011 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

The in origin intertidal marine brown alga Fucus vesiculosus L. grow permanently sublittoral in the brackish Bothnian Sea, side by side with the recently discovered F. radicans L. Bergström et L. Kautsky. Environmental conditions like salinity, light and temperature are clearly different between F. vesiculosus growth sites in the Bothnian Sea (4-5 practical salinity units, psu; part of the Baltic Sea) and the tidal Norwegian Sea (34-35 psu; part of the Atlantic Ocean). The general aims of this thesis were to compare physiological aspects between the marine ecotype and the brackish ecotype of F. vesiculosus as well as between the two Bothnian Sea species F. vesiculosus and F. radicans.

The result in the study indicates a higher number of water soluble organic compounds in the marine ecotype of F. vesiculosus compared to the brackish ecotype. These compounds are suggested to be compatible solutes and be due to an intertidal and sublittoral adaptation, respectively; where the intertidal ecotype needs the compounds as a protection from oxygen radicals produced during high irradiation at low tide. The sublittoral ecotype might have lost the ability to synthesize these compound/compounds due to its habitat adaptation. The mannitol content is also higher in the marine ecotype compared to the brackish ecotype of F. vesiculosus and this is suggested to be due to both higher level of irradiance and higher salinity at the growth site.

77 K fluorescence emission spectra and immunoblotting of D1 and PsaA proteins indicate that both ecotypes of F. vesiculosus as well as F. radicans have an uneven ratio of photosystem II/photosystem I (PSII/PSI) with an overweight of PSI. The fluorescence emission spectrum of the Bothnian Sea ecotype of F. vesiculosus however, indicates a larger light-harvesting antenna of PSII compared to the marine ecotype of F. vesiculosus and F. radicans. Distinct differences in 77 K fluorescence emission spectra between the Bothnian Sea ecotype of F. vesiculosus and F. radicans confirm that this is a reliable method to use to separate these species.

The marine ecotype of F. vesiculosus has a higher photosynthetic maximum (Pmax) compared to the brackish ecotype of F. vesiculosus and F. radicans whereas both the brackish species have similar Pmax. A reason for higher Pmax in the marine ecotype of F. vesiculosus compared to F. radicans is the greater relative amount of ribulose-1.5-bisphosphate carboxylase/oxygenase (Rubisco). The reason for higher Pmax in marine ecotype of F. vesiculosus compare to the brackish ecotype however is not due to the relative amount of Rubisco and further studies of the rate of CO2 fixation by Rubisco is recommended. Treatments of the brackish ecotype of F. vesiculosus in higher salinity than the Bothnian Sea natural water indicate that the most favourable salinity for high Pmax is 10 psu, followed by 20 psu. One part of the explanation to a high Pmax in 10 psu is a greater relative amount of PsaA protein in algae treated in 10 psu. The reason for greater amount of PsaA might be that the algae need to produce more ATP, and are able to have a higher flow of cyclic electron transport around PSI to serve a higher rate of CO2 fixation by Rubisco. However, studies of the rate of CO2 fixation by Rubisco in algae treated in similar salinities as in present study are recommended to confirm this theory.

 

Abstract [sv]

Fucus vesiculosus L. (Blåstång) är en brunalg som i huvudsak växer i tidvattenzonen i marint vatten men arten klarar också att växa konstant under ytan i det bräckta Bottenhavet. Norska havet och den del av Bottenhavet, där algerna är insamlade i denna studie, har salthalterna 34-35 psu (praktisk salthaltsenhet) respektive 4-5 psu. F. radicans L. Bergström et L. Kautsky (Smaltång) är en nyligen upptäckt art (2005) som har utvecklats i Bottenhavet. F. radicans och Bottenhavets ekotyp av F. vesiculosus växer sida vid sida och har tidigare ansetts vara samma art. Sett till hela Östersjön, så ändras ytans salthalt från 25 till 1-2 psu mellan Östersjöns gräns mot Kattegatt och norra Bottenviken. Den låga salthalten i Östersjön beror på det höga flödet av sötvatten från älvarna och på ett litet inflödet av saltvatten i inloppet vid Kattegatt. Salthaltsgradienten är korrelerad med antalet arter som minskar med minskad salthalt. Östersjön är ett artfattigt hav och de arter som finns är till stor del en blandning av söt- och saltvattenarter. Det finns bara ett fåtal arter som är helt anpassade till bräckt vatten och F. radicans är en av dem. Exempel på miljöskillnader för F. vesiculosus i Norska havet och i Bottenhavet är salthalten, tidvattnet, ljuset och temperaturen. Tidvattnet i Norska havet gör att algerna växlar mellan att vara i vattnet och på land, vilket utsätter algerna för stora ljusskillnader, snabba och stora temperaturväxlingar samt även torka. De alger som växer i Bottenhavet har däremot en jämnare och lägre temperatur, istäcke på vintern och mindre tillgång på ljus eftersom de alltid lever under vattenytan. Skillnaderna i miljön mellan växtplatserna leder till skillnader i fysiologiska anpassningar. Anledningen till att F. vesiculosus och F. radicans valdes som studieobjekt i denna avhandling är att de är viktiga nyckelarter i Bottenhavet. F. vesiculosus och F. radicans är de enda större bältesbildande alger som finns i det artfattiga ekosystemet och de används därför flitigt som mat, gömställe, parningsplats och barnkammare för t.ex. fisk. Att de är nyckelarter gör det angeläget att försöka förstå hur algerna är anpassade och hur de reagerar på miljöförändringar för att få veta hur de kan skyddas och bevaras. F. radicans inkluderades även för att se hur en naturlig art i Bottenhavet är anpassad i jämförelse med den invandrade F. vesiculosus. Marin F. vesiculosus inkluderades för att vara en artreferens från artens naturliga växtplats.

Studien visar att det finns fler vattenlösliga organiska substanser (finns vissa organiska substanser som har en proteinskyddande funktion) i den marina ekotypen av of F. vesiculosus än i Bottenhavets ekotyp. Anledningen till detta föreslås vara en anpassning till att växa i tidvattenzonen. Vid lågvatten utsätts F. vesiculosus från Norska havet för starkt ljus, uttorkning, och snabba temperatur- växlingar vilket gör att den kan behöva dessa organiska substanser som skydd mot fria syreradikaler som bildas under lågvattenexponeringarna. F. vesiculosus från Bottenhavet har troligen mist förmågan att syntetisera dessa substanser på grund av anpassning till att hela tiden växa under ytan. Mängden mannitol (socker) är högre i den marina ekotypen av of F. vesiculosus än i Bottenhavets ekotyp. Detta föreslås bero på högre fotosyntetiskt maximum i F. vesiculosus från Norska havet jämfört med ekotypen från Bottenhavet. Skillnaden i fotssyntetiskt maximum är bland annat kopplat till ljus- och salthaltskillnaden på algernas växtplatser. Denna teori styrks av att både fotosyntesen och halten av mannitol ökar i Bottenhavets ekotyp när den behandlas i högre salthalt.

Studien visar även att båda ekotyperna av F. vesiculosus samt F. radicans har ett ojämnt förhållande mellan fotosystem II och I (PSII och PSI) med en dominans av PSI. Denna slutsats är baserad på fluorescens emissions mätningar vid 77 K (-196 °C) och mätning av den relativa mängden D1 protein (motsvarar PSII) och PsaA protein (motsvarar PSI). F. vesiculosus från Bottenhavet visar ett emission spektrum som pekar mot en jämnare fördelning av PSII och PSI jämfört med den marina ekotypen och F. radicans. Detta stämmer dock inte med förhållandet mellan D1/PsaA som indikerar att alla tre har mer PSI än PSII. Förklaringen till avvikelsen mellan metoderna antas vara att F. vesiculosus från Bottenhavet har större ljus-infångande antennpigment än marin F. vesiculosus och F. radicans. De tydliga skillnaderna i 77 K fluorescens emission spektra mellan Bottenhavets F. vesiculosus och F. radicans visar att denna metod kan användas som säker artidentifiering.

Den marina ekotypen av F. vesiculosus har högre fotosyntetiskt maximum än de båda arterna från Bottenhavet. Mätningar av den relativa mängden av enzymet Rubisco, viktigt för upptaget av koldioxid hos växter och alger, visar att mängden enzym är en sannolik förklaring till skillnaden i fotosyntetiskt maximum mellan den marina ekotypen av F. vesiculosus och F. radicans och detta är troligen en normal artskillnad. Mängden Rubisco kan dock inte förklara skillnaden i fotosyntetiskt maximum mellan de båda ekotyperna av F. vesiculosus. För att undersöka vad skillnaden mellan dessa två beror på så föreslås istället mätningar av Rubisco’s koldioxidfixeringshastighet.

Det är en ökning av fotosyntetiskt maximum i Bottenhavets ekotyp av F. vesiculosus när den behandlas i högre salthalt (10, 20 och 35 psu) och det högsta fotosyntetiska maximumet uppmättes i alger som behandlats i 10 psu. Denna ökning beror inte på ökning i den relativa mängden av Rubisco. Ökningen i fotosyntesen speglas dock av en ökning av den relativa mängden PsaA. Detta antas bero på att det behövs mer energi i form av ATP och att en ökning av detta kan ske på grund av att mer PsaA kan driva den cykliska elektrontransporten i fotosyntesreaktionen. Ökat behov av ATP antas bero på en ökning av Rubisco aktiviteten men mätning av aktiviteten krävs för att bekräfta detta.

 

Place, publisher, year, edition, pages
Sundsvall: Kopieringen Mid Sweden University, 2011. p. 62
Series
Mid Sweden University doctoral thesis, ISSN 1652-893X ; 102
Keywords
Bothnian Sea, brackish, brown algae, D1, 77 K fluorescence emission, Fucus vesiculosus, Fucus radicans, light-harvest antenna, mannitol, marine, NMR, Norwegian Sea, quantum yield, photosynthetic maximum capacity (Pmax), photosystem, (PSI, PSII), PsaA, Rubisco, salinity.
National Category
Biological Sciences Botany
Identifiers
urn:nbn:se:miun:diva-13308 (URN)978-91-86694-25-8 (ISBN)
Public defence
2011-03-25, Mittuniversitetet sal O102, Holmgatan 10, 851 70 Sundsvall, 10:15 (Swedish)
Opponent
Supervisors
Available from: 2011-02-25 Created: 2011-02-24 Last updated: 2013-01-30Bibliographically approved
Gylle, A. M., Nygård, C. A. & Ekelund, N. G. (2009). Desiccation and salinity effects on marine and brackhish Fucus vesiculosus L. (Phaeophyceae).. Phycologia, 48(3), 156-164
Open this publication in new window or tab >>Desiccation and salinity effects on marine and brackhish Fucus vesiculosus L. (Phaeophyceae).
2009 (English)In: Phycologia, ISSN 0031-8884, E-ISSN 2330-2968, Vol. 48, no 3, p. 156-164Article in journal (Other academic) Published
Abstract [en]

Ecotypes of Fucus vesiculosus L. from the Norwegian Sea (34 psu, marine ecotype) and Bothnian Sea (5 psu, brackish ecotype) have been compared with respect to the ability to withstand desiccation at different temperatures (0, 10, and 20°C). The aim was also to investigate the importance of salinity and light for the availably energy reserves, osmotic adjustment, and pigment content. The maximum quantum yield of photosystem II photochemistry (Fv/Fm) values revealed that the marine ecotype was more able to resist desiccation. The brackish algae showed a decrease in Fv/Fm as a response to desiccation at all temperatures, but the decrease was most pronounced at 20°C. The brackish ecotype recovered from desiccation within 5 h only when treated at 0°C. When the two ecotypes were treated at different levels of salinity in darkness and light, the results suggested that both salinity and irradiance are main factors in the differences in mannitol content between the two ecotypes. Chlorophyll (Chl) measurements showed 25% higher Chl α and 60% higher Chl c in the brackish ecotype in comparison to the marine ecotype. Darkness had a more pronounced effect on the Chl content than the salinity and initiated an increase in the amount of Chl, especially Chl c in the brackish ecotype.

Keywords
Brackish, Chlorophyll, Emersion, Mannitol, Marine, Photosynthetic maximal quantum yield (Fv/Fm)
National Category
Ecology
Identifiers
urn:nbn:se:miun:diva-8845 (URN)10.2216/08-45.1 (DOI)000265871800002 ()2-s2.0-66149182906 (Scopus ID)
Available from: 2009-05-06 Created: 2009-05-06 Last updated: 2017-12-13Bibliographically approved
Gylle, M., Isaksson, D. & Ekelund, N. (2009). Research note: Ecotype differentiation in qualitative content of water soluble organic compounds between marine and brackish Fucus vesiculosus L. (Phaeophyceae). Phycological Research, 57(2), 127-130
Open this publication in new window or tab >>Research note: Ecotype differentiation in qualitative content of water soluble organic compounds between marine and brackish Fucus vesiculosus L. (Phaeophyceae)
2009 (English)In: Phycological Research, ISSN 1322-0829, E-ISSN 1440-1835, Vol. 57, no 2, p. 127-130Article in journal (Refereed) Published
Abstract [en]

In the present study we compared the contents of water soluble organic compounds of the marine intertidal ecotype of Fucus vesiculosus (Phaeophyceae) from the Norwegian Sea (34 practical salinity units, psu) with the sublittoral ecotype of F. vesiculosus from the brackish Bothnian Sea (5 psu). Nuclear magnetic resonance spectra revealed that marine F. vesiculosus had additional types of water soluble organic compounds compared with brackish F. vesiculosus. The results suggested that glycine betaine in the marine ecotype could be the reason for this ecotype differentiation. Furthermore, the qualitative differences between the ecotypes were the same after one week's treatment of marine algae in brackish water and of brackish algae in marine water. These suggest that the additional types of water soluble organic compounds in marine F. vesiculosus are not caused by the salinity conditions at the growth sites. Further research concerning other environmental factors that may influence ecotype differentiation of water soluble organic compounds qualitative content and adaptation in F. vesiculosus is recommended.

Keywords
adaptation; Bothnian Sea; C-13 NMR; glycine betaine; mannitol;
National Category
Biological Sciences
Identifiers
urn:nbn:se:miun:diva-7149 (URN)10.1111/j.1440-1835.2009.00529.x (DOI)000266431200006 ()2-s2.0-66149153883 (Scopus ID)
Available from: 2008-11-29 Created: 2008-11-23 Last updated: 2017-12-14Bibliographically approved
Ekelund, N. G. A., Nygård, C. A., Nordström, R. & Gylle, M. (2008). In situ study of relative electron transport rates (ETR) in the marine macroalga Fucus vesiculosus from the Baltic Sea at different depths and times of the year.. Journal of Applied Phycology, 20(5), 751-756
Open this publication in new window or tab >>In situ study of relative electron transport rates (ETR) in the marine macroalga Fucus vesiculosus from the Baltic Sea at different depths and times of the year.
2008 (English)In: Journal of Applied Phycology, ISSN 0921-8971, E-ISSN 1573-5176, Vol. 20, no 5, p. 751-756Article in journal (Refereed) Published
Abstract [en]

The brown alga Fucus vesiculous is one of the few marine species in the Baltic Sea. F. vesiculosus shows high morphological and physiological variability as a response to its environmental conditions. The salinity in the Baltic Sea is 4-5 psu in comparison to 35 psu in the Atlantic. Photosynthesis of algae is usually measured after collection and transportation to constant culture conditions. However, in this study the relative photosynthetic electron transport rate (rETR), calculated from chl a fluorescence parameters were compared for algae from 1 and 4 m depths by SCUBA divers. Measurements of light response curves from the same individuals of F. vesiculosus at different depths and times of the year have to our knowledge never been made before. Measurements were performed at four different occasions during the spring (February 25, April 3 and 29 and May 26 in 2005) in the Baltic Sea, using rapid light curves (RLCs) generated with a Diving PAM. In addition, samples were collected for photoinhibition studies in the laboratory. The light response curves obtained in situ at 1 and 4 m depths for F. vesiculosus showed lower values of light saturation with depth. When algae from 1 and 4 m depths were exposed to high irradiances of PAR (1400 µmol photons m-2 s-1), algae from 1 m depth showed a higher degree of photoinhibition in comparison to algae from the 4 m depth.

Keywords
Diving PAM, fluorescence, photoinhibition, photosynthesis.
National Category
Biological Sciences
Identifiers
urn:nbn:se:miun:diva-6528 (URN)10.1007/s10811-007-9288-y (DOI)000261969300038 ()2-s2.0-58149250905 (Scopus ID)5391 (Local ID)5391 (Archive number)5391 (OAI)
Available from: 2008-12-07 Created: 2008-12-07 Last updated: 2017-12-12Bibliographically approved
Gylle, M. (2007). Physiological responses of marine and brackish Fucus vesiculosus L. with respect to salinity. (Licentiate dissertation). Sundsvall: Mittuniversitetet
Open this publication in new window or tab >>Physiological responses of marine and brackish Fucus vesiculosus L. with respect to salinity
2007 (English)Licentiate thesis, comprehensive summary (Other academic)
Abstract [en]

The intertidal brown alga Fucus vesiculosus L. is mainly a marine species (34 practical salinity units, psu), but the alga also grows in the sublittoral of the brackish Bothnian Sea (part of the Baltic Sea; 5 psu). The conditions at the growth sites are clearly different between the Bothnian Sea and the Norwegian Sea (part of Atlantic) with constant low salinity and a lack of tides in the Bothnian Sea. The objectives of the thesis were to compare the physiology in marine and brackish ecotypes of F. vesiculosus with respect to salinity and the ability of F. vesiculosus to acclimate to different salinities. A study of photosynthetic maximum capacity and relative amount of Rubisco in relation to salinity in brackish F. vesiculosus were also performed. The results showed that both ecotypes of F. vesiculosus have the same potential to use the available excitation energy for photochemistry. The results also suggest that this is relatively independent of salinity changes. There were a higher number of water soluble organic compounds, higher mannitol content (mmol kg‐1 DW), lower chlorophyll (Chl) content (mg g‐1 DW) and higher tolerance to desiccation in the marine ecotype. The number of water soluble carbon compounds did not change when the algae were treated to either high or low salinities and it was suggested that the differences were due to an intertidal or sublittoral acclimation, and not salinity. Both ecotypes showed changed mannitol content as a response to changed salinity but the changes were different between the ecotypes and seasons. The content of mannitol and the osmotic adjustment by mannitol in a longer timescale than 24 h appears to be closely connected to irradiance and photosynthesis in addition to the salinity. The main reason for higher rate of photosynthesis in higher salinity for the brackish ecotype is not clarified because no correlation could be detected between photosynthesis and the relative amount of Rubisco. The Chl content increased in darkness and the differences between the ecotypes are probably due to a compensation for low irradiance in the sublittoral growth site. Higher tolerance for desiccation in marine ecotype was concluded to be due to a lower rate of water loss because of more mannitol and thicker thallus.

Place, publisher, year, edition, pages
Sundsvall: Mittuniversitetet, 2007. p. 26
Series
Mid Sweden University licentiate thesis, ISSN 1652-8948 ; 19
Keywords
acclimation, Bothnian Sea, compatible solutes, desiccation, fluorescence, F. vesiculosus, F. radicans, immunoblot, mannitol, photosynthesis, Rubisco, salinity, Algfysiologi
National Category
Biological Sciences
Identifiers
urn:nbn:se:miun:diva-9329 (URN)978-91-85317-41-7 (ISBN)
Presentation
(English)
Available from: 2009-07-10 Created: 2009-07-10 Last updated: 2009-07-13Bibliographically approved
Gylle, A. M., Nygård, C. A., Pocock, T. & Ekelund, N. G. A. (2007). Salinity effect on oxygen evolution and the relative amount of RuBisCO in the brackish Fucus vesiculosus L. (Phaeophyceae). In: Pysiological responses of marine and brackish Fucuc vesiculosus L with respect to salinity (pp. 1-8). Sundsvall: Mittuniversitetet
Open this publication in new window or tab >>Salinity effect on oxygen evolution and the relative amount of RuBisCO in the brackish Fucus vesiculosus L. (Phaeophyceae)
2007 (English)In: Pysiological responses of marine and brackish Fucuc vesiculosus L with respect to salinity, Sundsvall: Mittuniversitetet , 2007, p. 1-8Chapter in book (Other academic)
Abstract [en]

The sublittoral Fucus vesiculosus from the brackish Bothnian Sea is adapted to a salinity of 4-5 practical salinity units (psu). This study investigated the effect of different salinities (5, 10, 20 and 35 psu) on maximum photosynthetic capacity (Pmax) and the relative amount of ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco). The results revealed a higher Pmax at higher salinities with the maximum at 10 psu. Higher salinities also resulted in increased relative amounts of Rubisco but this was not well correlated with the increased Pmax. Therefore, the amount of Rubisco doesn�t appear to be the main reason for the increased Pmax in higher salinities.

Place, publisher, year, edition, pages
Sundsvall: Mittuniversitetet, 2007
Series
Mid Sweden University Licentiate Thesis, ISSN 1652-8948 ; 19 [Paper IV]
Keywords
brackish, oxygen evolution, photosynthesis, Rubisco, salinity, Fucus vesiculosus immunoblotting.
National Category
Biological Sciences
Identifiers
urn:nbn:se:miun:diva-5936 (URN)4912 (Local ID)978-91-85317-41-7 (ISBN)4912 (Archive number)4912 (OAI)
Available from: 2008-09-30 Created: 2008-09-30 Last updated: 2013-01-10Bibliographically approved
Gylle, A. M., Isaksson, D. & Ekelund, N. (2005). THE CONCENTRATIONS OF MANNITOL IN FUCUS VESICULOSUS AS A RESPONSE TO DIFFERENT SALINITIES. In: PHYCOLOGIA (pp. 42-42).
Open this publication in new window or tab >>THE CONCENTRATIONS OF MANNITOL IN FUCUS VESICULOSUS AS A RESPONSE TO DIFFERENT SALINITIES
2005 (English)In: PHYCOLOGIA, 2005, p. 42-42Conference paper, Published paper (Refereed)
Series
PHYCOLOGIA, ISSN 0031-8884 ; 44-4
Identifiers
urn:nbn:se:miun:diva-11866 (URN)000205501500100 ()
Available from: 2010-07-15 Created: 2010-07-15 Last updated: 2011-04-06Bibliographically approved
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