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Publications (10 of 110) Show all publications
Chudinova, E. A., Surmeneva, M. A., Timin, A. S., Karpov, T. E., Wittmar, A., Ulbricht, M., . . . Surmenev, R. A. (2019). Adhesion, proliferation, and osteogenic differentiation of human mesenchymal stem cells on additively manufactured Ti6Al4V alloy scaffolds modified with calcium phosphate nanoparticles. Colloids and Surfaces B: Biointerfaces, 176, 130-139
Open this publication in new window or tab >>Adhesion, proliferation, and osteogenic differentiation of human mesenchymal stem cells on additively manufactured Ti6Al4V alloy scaffolds modified with calcium phosphate nanoparticles
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2019 (English)In: Colloids and Surfaces B: Biointerfaces, ISSN 0927-7765, E-ISSN 1873-4367, Vol. 176, p. 130-139Article in journal (Refereed) Published
Abstract [en]

In the present study, biocomposites based on 3D porous additively manufactured Ti6Al4V (Ti64) scaffolds modified with biocompatible calcium phosphate nanoparticles (CaPNPs) were investigated. Ti64 scaffolds were manufactured via electron beam melting technology using an Arcam machine. Electrophoretic deposition was used to modify the scaffolds with CaPNPs, which were synthesized by precipitation in the presence of polyethyleneimine (PEI). Dynamic light scattering revealed that the CaP/PEI nanoparticles had an average size of 46 ± 18 nm and a zeta potential of +22 ± 9 mV. Scanning electron microscopy (SEM) revealed that the obtained spherical CaPNPs had an average diameter of approximately 90 nm. The titanium-based scaffolds coated with CaPNPs exhibited improved hydrophilic surface properties, with a water contact angle below 5°. Cultivation of human mesenchymal stem cells (hMSCs) on the CaPNPs-coated Ti64 scaffolds indicated that the improved hydrophilicity was beneficial for the attachment and growth of cells in vitro. The Ti6Al4V/CaPNPs scaffold supported an increase in the alkaline phosphatase (ALP) activity of cells. In addition to the favourable cell proliferation and differentiation, Ti6Al4V/CaPNPs scaffolds displayed increased mineralization compared to non-coated Ti6Al4V scaffolds. Thus, the developed composite 3D scaffolds of Ti6Al4V functionalized with CaPNPs are promising materials for different applications related to bone repair. 

Keywords
Additive manufacturing, Calcium phosphate, Cell adhesion, Electron beam melting, Electrophoretic deposition, Nanoparticles, Proliferation in vivo, Scaffold, Surface properties
Identifiers
urn:nbn:se:miun:diva-35415 (URN)10.1016/j.colsurfb.2018.12.047 (DOI)2-s2.0-85059137646 (Scopus ID)
Available from: 2019-01-10 Created: 2019-01-10 Last updated: 2019-01-10Bibliographically approved
Petrone, N., Carraro, G., Dal Castello, S., Broggio, L., Koptioug, A. & Bäckström, M. (2018). A Novel Instrumented Human Head Surrogate For The Impact Evaluation Of Helmets. In: Dr Hugo Espinosa, David R. Rowlands, Jonathan Shepherd, Professor David Thiel (Ed.), Proceedings, Volume 2, ISEA 2018: . Paper presented at 12th Conference on the Engineering of Sport, Brisbane, Australia, 26 to 29 March 2018 (pp. 269). , 2, Article ID 6.
Open this publication in new window or tab >>A Novel Instrumented Human Head Surrogate For The Impact Evaluation Of Helmets
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2018 (English)In: Proceedings, Volume 2, ISEA 2018 / [ed] Dr Hugo Espinosa, David R. Rowlands, Jonathan Shepherd, Professor David Thiel, 2018, Vol. 2, p. 269-, article id 6Conference paper, Published paper (Refereed)
Abstract [en]

A novel Human Head Surrogate was obtained from available MRI scans of a 50th percentile male human head. Addictive manufacturing was used to produce the skull, the brain and the skin. All original MRI geometries were partially smoothed and adjusted to provide the best biofidelity compatible with printing and molding technology. The skull was 3D-printed in ABS and ten pressure sensors were placed into it. The brain surrogate was cast from silicon rubber in the 3d-printed plastic molds. Nine tri-axial accelerometers (placed at the tops of the lobes, at the sides of the lobes, in the cerebellum and in the center of mass) and a three-axis gyroscope (at the center of mass) were inserted into the silicon brain during casting. The cranium, after assembly with brain, was filled with silicon oil mimicking the cerebral fluid. Silicon rubber was cast in additional 3d-printed molds to form the skin surrounding the cranium. The skull base was adapted to be compatible with the Hybrid-III neck and allow the exit of brain sensors cabling. Preliminary experiments were carried out proving the functionality of the surrogate. Results showed how multiple accelerometers and pressure sensors allowed a better comprehension of the head complex motion during impacts.

Keywords
human head, surrogate, additive manufacturing, accelerometers, impact tests
National Category
Medical Laboratory and Measurements Technologies Other Materials Engineering Other Mechanical Engineering Interaction Technologies
Identifiers
urn:nbn:se:miun:diva-32502 (URN)10.3390/proceedings2060269 (DOI)
Conference
12th Conference on the Engineering of Sport, Brisbane, Australia, 26 to 29 March 2018
Projects
STII
Funder
VINNOVA
Available from: 2017-12-19 Created: 2017-12-19 Last updated: 2018-04-25Bibliographically approved
Koptioug, A., Bäckström, M., Botero Vega, C. A., Popov, V. & Chudinova, E. (2018). Developing new materials for Electron Beam Melting: experiences and challenges. In: : . Paper presented at THERMEC'2018 International Conference on Processing and Manufacturing of Advanced Materials Processing, Fabrication, Properties, Applications, Paris, France July 8-13, 2018.
Open this publication in new window or tab >>Developing new materials for Electron Beam Melting: experiences and challenges
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2018 (English)Conference paper, Published paper (Refereed)
Abstract [en]

Lack of industrially available materials for additive manufacturing (AM) of metallic materials along with the promises of materials with improved or unique properties provides a strong drive for developing new process/material combinations. As powder bed technologies for metallic materials are relatively new to the market, and to some extent are only maturing, developers of new process/material combinations have certain challenges to overcome. Firstly, basic knowledge on the behavior of materials (even those well established for other applications) under extreme conditions of melting/solidification with beam-based AM methods is far from being adequate. Secondly, manufacturing of the equipment is up to date driven by industrial application, thus optimization of the AM machines for small test batches of powders is still belongs to research and development projects. Also, majority of the powder manufacturers are primarily driven by the market development, and even they are well aware of the demands imposed by the powder bed AM machines, availability of small test batches of adequate powders may be problematic or at least quite costly for the R&D oriented users. Present paper describes the experiences in developing new materials for EBM A2 machine by Arcam EBM, modified for operating with powder batches of 100-200 ml and less. In particular it discusses achievements and challenges of working with powders from different materials with specifications far beyond the range suggested by machine manufacturer. Also it discusses the possibility of using blended rather than pre-alloyed powders for achieving both composite-like and alloyed materials in the same part by steering electron beam energy deposition strategy.

Keywords
Additive Manufacturing, Electron Beam Melting, material development, blended powders, in situ alloying, composite materials
National Category
Metallurgy and Metallic Materials
Identifiers
urn:nbn:se:miun:diva-35136 (URN)
Conference
THERMEC'2018 International Conference on Processing and Manufacturing of Advanced Materials Processing, Fabrication, Properties, Applications, Paris, France July 8-13, 2018
Note

Paper accepted, in print

Available from: 2018-12-10 Created: 2018-12-10 Last updated: 2018-12-13Bibliographically approved
Pushilina, N., Panin, A., Syrtanov, M., Kashkarov, E., Kudiiarov, V., Perevalova, O., . . . Koptioug, A. (2018). Hydrogen-induced phase transformation and microstructure evolution for Ti-6Al-4V parts produced by electron beam melting. Metals, 8(5), Article ID 301.
Open this publication in new window or tab >>Hydrogen-induced phase transformation and microstructure evolution for Ti-6Al-4V parts produced by electron beam melting
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2018 (English)In: Metals, ISSN 2075-4701, Vol. 8, no 5, article id 301Article in journal (Refereed) Published
Abstract [en]

In this paper, phase transitions and microstructure evolution in titanium Ti-6Al-4V alloy parts produced by electron beam melting (EBM) under hydrogenation was investigated. Hydrogenation was carried out at the temperature of 650 °C to the absolute hydrogen concentrations in the samples of 0.29, 0.58, and 0.90 wt. %. Comparative analysis of microstructure changes in Ti-6Al-4V alloy parts was performed using scanning electron microscopy (SEM), transmission electron microscopy (TEM), and X-ray diffraction (XRD). Furthermore, in-situ XRD was used to investigate the phase transitions in the samples during hydrogenation. The structure of Ti-6Al-4V parts produced by EBM is represented by the α phase plates with the transverse length of 0.2 µm, the β phase both in the form of plates and globular grains, and metastable α'' and ω phases. Hydrogenation to the concentration of 0.29 wt. % leads to the formation of intermetallic Ti3Al phase. The dimensions of intermetallic Ti3Al plates and their volume fraction increase significantly with hydrogen concentration up to 0.58 wt. % along with precipitation of nano-sized crystals of titanium δ hydrides. Individual Ti3Al plates decay into nanocrystals with increasing hydrogen concentration up to 0.9 wt. % accompanied by the increase of proportion and size of hydride plates. Hardness of EBM Ti-6Al-4V alloy decreases with hydrogen content. 

Keywords
Additive manufacturing, Electron beam melting, Hydrogen, Microstructure, Phase transitions, Titanium Ti-6Al-4V alloy
National Category
Other Mechanical Engineering
Identifiers
urn:nbn:se:miun:diva-33739 (URN)10.3390/met8050301 (DOI)000435109300011 ()2-s2.0-85046696127 (Scopus ID)
Available from: 2018-06-10 Created: 2018-06-10 Last updated: 2018-08-10Bibliographically approved
Pushilina, N., Syrtanov, M., Kashkarov, E., Murashkina, T., Kudiiarov, V., Laptev, R., . . . Koptioug, A. (2018). Influence of Manufacturing Parameters on Microstructure and Hydrogen Sorption Behavior of Electron Beam Melted Titanium Ti-6Al-4V Alloy. Materials, 11(5), Article ID 763.
Open this publication in new window or tab >>Influence of Manufacturing Parameters on Microstructure and Hydrogen Sorption Behavior of Electron Beam Melted Titanium Ti-6Al-4V Alloy
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2018 (English)In: Materials, ISSN 1996-1944, E-ISSN 1996-1944, Vol. 11, no 5, article id 763Article in journal (Refereed) Published
Abstract [en]

Influence of manufacturing parameters (beam current from 13 to 17 mA, speed function 98 and 85) on microstructure and hydrogen sorption behavior of electron beam melted (EBM) Ti-6Al-4V parts was investigated. Optical and scanning electron microscopies as well as X-ray diffraction were used to investigate the microstructure and phase composition of EBM Ti-6Al-4V parts. The average alpha lath width decreases with the increase of the speed function at the fixed beam current (17 mA). Finer microstructure was formed at the beam current 17 mA and speed function 98. The hydrogenation of EBM Ti-6Al-4V parts was performed at the temperatures 500 and 650 degrees C at the constant pressure of 1 atm up to 0.3 wt %. The correlation between the microstructure and hydrogen sorption kinetics by EBM Ti-6Al-4V parts was demonstrated. Lower average hydrogen sorption rate at 500 degrees C was in the sample with coarser microstructure manufactured at the beam current 17 mA and speed function 85. The difference of hydrogen sorption kinetics between the manufactured samples at 650 degrees C was insignificant. The shape of the kinetics curves of hydrogen sorption indicates the phase transition alpha(H)+beta(H)->beta(H).

National Category
Mechanical Engineering
Identifiers
urn:nbn:se:miun:diva-34106 (URN)10.3390/ma11050763 (DOI)000434711700113 ()29747471 (PubMedID)2-s2.0-85046844088 (Scopus ID)
Available from: 2018-07-04 Created: 2018-07-04 Last updated: 2018-07-04Bibliographically approved
Olsén, J., Shen, Z., Liu, L., Koptyug, A. & Rännar, L.-E. (2018). Micro- and macro-structural heterogeneities in 316L stainless steel prepared by electron-beam melting. Materials Characterization, 141, 1-7
Open this publication in new window or tab >>Micro- and macro-structural heterogeneities in 316L stainless steel prepared by electron-beam melting
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2018 (English)In: Materials Characterization, ISSN 1044-5803, E-ISSN 1873-4189, Vol. 141, p. 1-7Article in journal (Refereed) Published
Abstract [en]

This is a study of the micro- and macrostructural variations in samples of stainless steel with the overall composition of the grade 316L, produced using electron beam melting. Electron beam melting is one of the processing methods under consideration for manufacturing some of the International Thermo- Nuclear Experimental Reactor In-Vessel components. Therefore further studies of the homogeneity of the material were conducted. Electron beam melting results in a complicated thermal history of the manufactured part giving a significant impact on the microstructure. A cellular structure that is often observed in samples prepared by selective laser melting was found in the top layers of the specimens. Further down, the structure changed until the cellular structure was almost non-existing, and the grain boundaries had become more pronounced. This revelation of a heterogeneous structure throughout the entire part is crucial for large-scale industrial applications like the Thermo- Nuclear Experimental Reactor to make sure that it is understood that the properties of the material might not be the same at every point, as well as to assure that the correct post-treatment is done. It is also exposed that a significant part of this change is due to molybdenum redistribution inside the sample when it diffuses from the cell boundaries into the cells, and into bigger agglomerates in the grain boundaries. This diffusion seems not to affect the microhardness of the samples. 

Keywords
316L stainless steel, Additive manufacturing, Electron beam melting, Heterogeneous material, Microstructure
National Category
Other Mechanical Engineering
Identifiers
urn:nbn:se:miun:diva-33692 (URN)10.1016/j.matchar.2018.04.026 (DOI)000435428100001 ()2-s2.0-85046110254 (Scopus ID)
Available from: 2018-06-01 Created: 2018-06-01 Last updated: 2018-08-10Bibliographically approved
Botero, C. A., Koptyug, A., Jiménez-Piqué, E. & Rännar, L.-E. (2018). Microstructure and nanomechanical behavior of modified 316L-based materials fabricated using EBM. In: : . Paper presented at 2:nd International Conference on Electron Beam Additive Manufacturing, Nuremberg, Germany, April 11, 2018.
Open this publication in new window or tab >>Microstructure and nanomechanical behavior of modified 316L-based materials fabricated using EBM
2018 (English)Conference paper, Oral presentation with published abstract (Refereed)
Abstract [en]

Stainless steel 316L based materials modified by the additions of iron-based wear-resistant alloys (Colferoloy@ 103 and 139) used for thermal spray coatings applications were fabricated by EBM. Process parameters were tailored to fabricate compact specimens of 1cm3 in an Arcam A2 (Arcam AB, Mölndal, Sweden) at Mid Sweden University. Microstructural features of the materials obtained were characterized by OM and SEM in polished and etched samples. Nanoindentation tests carried out at different penetration depths were performed on selected areas of the polished specimens to evaluate the materials micro/nano mechanical behavior and to establish correlations with the observed microstructure.

National Category
Other Materials Engineering
Identifiers
urn:nbn:se:miun:diva-35341 (URN)
Conference
2:nd International Conference on Electron Beam Additive Manufacturing, Nuremberg, Germany, April 11, 2018
Available from: 2018-12-21 Created: 2018-12-21 Last updated: 2019-01-10Bibliographically approved
Botero Vega, C. A., Jiménez-Piqué, E., Roos, S., Skoglund, P., Koptioug, A., Rännar, L.-E. & Bäckström, M. (2018). Nanoindentation: a suitable tool in metal Additive Manufacturing. In: : . Paper presented at Materials Science & Technology, MS&T 2018, October 14-18, Columbus, USA.
Open this publication in new window or tab >>Nanoindentation: a suitable tool in metal Additive Manufacturing
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2018 (English)Conference paper, Oral presentation only (Refereed)
National Category
Other Materials Engineering
Identifiers
urn:nbn:se:miun:diva-35342 (URN)
Conference
Materials Science & Technology, MS&T 2018, October 14-18, Columbus, USA
Available from: 2018-12-21 Created: 2018-12-21 Last updated: 2019-01-09Bibliographically approved
Douglas, T. E. L., Hempel, U., Żydek, J., Vladescu, A., Pietryga, K., Kaeswurm, J. A. H., . . . Pamuła, E. (2018). Pectin coatings on titanium alloy scaffolds produced by additive manufacturing: Promotion of human bone marrow stromal cell proliferation. Materials letters (General ed.), 227, 225-228
Open this publication in new window or tab >>Pectin coatings on titanium alloy scaffolds produced by additive manufacturing: Promotion of human bone marrow stromal cell proliferation
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2018 (English)In: Materials letters (General ed.), ISSN 0167-577X, E-ISSN 1873-4979, Vol. 227, p. 225-228Article in journal (Refereed) Published
Abstract [en]

Ti6Al4V is a popular biomaterial for load-bearing implants for bone contact, which can be fabricated by additive manufacturing technologies. Their long-term success depends on their stable anchoring in surrounding bone, which in turn depends on formation of new bone tissue on the implant surface, for which adhesion and proliferation of bone-forming cells is a pre-requisite. Hence, surface coatings which promote cell adhesion and proliferation are desirable. Here, Ti6Al4V discs prepared by additive manufacturing (EBM) were coated with layers of pectins, calcium-binding polysaccharides derived from citrus (C) and apple (A), which also contained alkaline phosphatase (ALP), the enzyme responsible for mineralization of bone tissue. Adhesion and proliferation of human bone marrow stromal cells (hBMSC) were assessed. Proliferation after 7 days was increased by A-ALP coatings and, in particular, by C-ALP coatings. Cell morphology was similar on coated and uncoated samples. In conclusion, ALP-loaded pectin coatings promote hBMSC adhesion and proliferation. 

Keywords
Biomaterials, Biomimetic, Thin films
National Category
Mechanical Engineering
Identifiers
urn:nbn:se:miun:diva-34113 (URN)10.1016/j.matlet.2018.05.060 (DOI)000436420200059 ()2-s2.0-85047304412 (Scopus ID)
Available from: 2018-07-04 Created: 2018-07-04 Last updated: 2018-08-13Bibliographically approved
Skoglund, P., Botero Vega, C. A., Koptioug, A., Rännar, L.-E. & Bäckström, M. (2018). Possibility of the “cold start” of the build in Electron Beam Melting. In: : . Paper presented at Materials Science & Technology, MS&T 2018, October 14-18, Columbus, USA.
Open this publication in new window or tab >>Possibility of the “cold start” of the build in Electron Beam Melting
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2018 (English)Conference paper, Oral presentation only (Refereed)
National Category
Other Mechanical Engineering
Identifiers
urn:nbn:se:miun:diva-35345 (URN)
Conference
Materials Science & Technology, MS&T 2018, October 14-18, Columbus, USA
Available from: 2018-12-21 Created: 2018-12-21 Last updated: 2019-01-09Bibliographically approved
Organisations
Identifiers
ORCID iD: ORCID iD iconorcid.org/0000-0003-2964-9500

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